Abstract
Of the Hemiptera, leafhoppers have been studied mainly in terms of cell cultures. The aim of culturing leafhopper cells is to study the relationship between leafhopper cells and plant viruses that persistently infect leafhopper vectors at the cellular level. Planthoppers and aphids are also vectors of plant viruses, and so cell cultures of these two groups of insects are desirable. However, unlike leafhoppers (Cicadellidae), planthoppers (Delphacidae) and aphids (Aphididae) are notoriously difficult to culture. For hemipteran cells, MM medium, Chiu and Black’s medium, or MGM-450 medium are used commonly (see Appendix 1).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Chiu, R. and Black, L.M (1967) Monolayer cultures of insect cell lines and their inoculation with a plant virus. Nature 215, 1076–1078.
Chiu, R. and Black, L.M (1969) Assay of wound tumor virus by the fluorescent cell counting technique. Virology 37, 667–677.
Kimura, I. (1984) Establishment of new cell lines from leafhopper vector and inoculation of its cell monolayers with rice dwarf virus. Proc. Jpn. Acad. 60B, 198–201.
Mitsuhashi, J. (1965) In vitro cultivation of the embryonic tissues of the green rice leafhopper, Nephotettix cincticeps Uhler (Homoptera: Cicadellidae). Jap. J. Appl. Entomol. Zool., 9, 107–114.
Mitsuhashi, J. (1969) Preliminary report on the primary culture of smaller brown planthopper cells in vitro. Appl. Entomol. Zool. 4, 151–153.
Mitsuhashi, J. (1979) Artificial rearing and aseptic rearing of leafhopper vectors: Applications in virus and MLO research. In: Maramorosch, K. and Harris, K.F. (eds) Leafhopper Vectors and Plant Disease Agents. Academic Press, New York, pp 369–412.
Mitsuhashi, J. and Maramorosch, K. (1963) Aseptic cultivation of four virus transmitting species of leafhoppers (Cicadellidae). Contrib. Boyce Thompson Inst. 22, 165–173.
Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435–460.
Peters, D. and Black, L.M (1971) Techniques for the cultivation of cells of the aphid Acyrthosiphon pisum in primary cultures. Tagungsbericht Deutsehe Akademie der Landwirtschaftswissenschaften zu Berlin, Nr. 115, 129–139.
Richardson, J. and Jensen, D.D. (1971) Tissue culture of monolayer cell lines of Colladonus montanus (Homoptera: Cicadellidae), a vector of the causal agent of western X-disease of peach. Ann. Entomol. Soc. Am. 64, 722–729.
Tokumitsu, T. and Maramorosch, K. (1966) Survival of aphid cells in vitro. Exp. Cell Res. 44, 652–655.
Yamada, K., Tokumitsu, T. and Shikata, E. (1970) Embryonic tissue culture of Laodelphax striatellus Fallén. Jap. J. Appl. Entomol. Zool. 14, 79–84 (in Japanese with English abstract).
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Japan
About this chapter
Cite this chapter
Mitsuhashi, J. (2002). Insecta (Hemiptera). In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_8
Download citation
DOI: https://doi.org/10.1007/978-4-431-67875-5_8
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-70313-6
Online ISBN: 978-4-431-67875-5
eBook Packages: Springer Book Archive