Of the Hemiptera, leafhoppers have been studied mainly in terms of cell cultures. The aim of culturing leafhopper cells is to study the relationship between leafhopper cells and plant viruses that persistently infect leafhopper vectors at the cellular level. Planthoppers and aphids are also vectors of plant viruses, and so cell cultures of these two groups of insects are desirable. However, unlike leafhoppers (Cicadellidae), planthoppers (Delphacidae) and aphids (Aphididae) are notoriously difficult to culture. For hemipteran cells, MM medium, Chiu and Black’s medium, or MGM-450 medium are used commonly (see Appendix 1).
KeywordsPhysiological Solution Thompson Inst Rice Dwarf Virus Leafhopper Vector Laodelphax Striatellus
Unable to display preview. Download preview PDF.
- Kimura, I. (1984) Establishment of new cell lines from leafhopper vector and inoculation of its cell monolayers with rice dwarf virus. Proc. Jpn. Acad. 60B, 198–201.Google Scholar
- Mitsuhashi, J. (1969) Preliminary report on the primary culture of smaller brown planthopper cells in vitro. Appl. Entomol. Zool. 4, 151–153.Google Scholar
- Mitsuhashi, J. (1979) Artificial rearing and aseptic rearing of leafhopper vectors: Applications in virus and MLO research. In: Maramorosch, K. and Harris, K.F. (eds) Leafhopper Vectors and Plant Disease Agents. Academic Press, New York, pp 369–412.Google Scholar
- Mitsuhashi, J. and Maramorosch, K. (1963) Aseptic cultivation of four virus transmitting species of leafhoppers (Cicadellidae). Contrib. Boyce Thompson Inst. 22, 165–173.Google Scholar
- Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435–460.Google Scholar
- Peters, D. and Black, L.M (1971) Techniques for the cultivation of cells of the aphid Acyrthosiphon pisum in primary cultures. Tagungsbericht Deutsehe Akademie der Landwirtschaftswissenschaften zu Berlin, Nr. 115, 129–139.Google Scholar
- Richardson, J. and Jensen, D.D. (1971) Tissue culture of monolayer cell lines of Colladonus montanus (Homoptera: Cicadellidae), a vector of the causal agent of western X-disease of peach. Ann. Entomol. Soc. Am. 64, 722–729.Google Scholar