Insecta (Coleoptera)

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Relatively few continuous cell lines have been established from Coleopteran insects. Culture media commonly used are modified IPL-52B, Schneider’s Drosophila medium, modified Grace’s medium, and MGM-450 medium (see Appendix 1).


Culture Vessel Physiological Solution Colorado Potato Beetle Insect Cell Line Continuous Cell Line 
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  1. Crawford, A.M (1982) A coleopteran cell line derived from Heteronychus arator (Coleoptera: Scarabaeidae). In Vitro 18, 813–816.CrossRefGoogle Scholar
  2. Lynn, D.E (1995) A new insect cell line from the Colorado potato beetle. In Vitro Cell. Dev. Biol. 31, 91–93.CrossRefGoogle Scholar
  3. Lynn, D.E. and Stoppleworth, A. (1984) Established cell lines from the beetle, Diabrotica undecimpunctata (Coleoptera: Chrysomelidae). In Vitro 20, 365–368.CrossRefGoogle Scholar
  4. Mitsuhashi, J. (1989) A continuous cell line from a beetle, Anomala costata Hope (Coleoptera, Scarabaeidae). J. Jpn. For. Soc. 71, 491–496.Google Scholar

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© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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