Of the dipteran insects, Drosophila melanogaster cell lines have been established in great number. However, reports on establishing D. melanogaster cell lines are relatively few, except for those cell lines established during the early stages of Drosophila cell culture history. Therefore, it is not known how many cell lines have been established from D. melanogaster. D. melanogaster cell lines have been established mostly for the study of cytogenetics. This is quite natural when we consider the accumulated genetic information on this insect. The second most common cell lines of Diptera have been derived from mosquitoes. Many cell lines have been established from various species of mosquitoes. The aim of establishing cell lines from mosquitoes is mostly to study arboviruses in vector cell culture with the hope of producing interferons. Other than D. melanogaster and mosquitoes, very few dipteran insects have been cultured.
KeywordsPrimary Culture Culture Vessel Physiological Solution Imaginal Disc Stainless Steel Mesh
Unable to display preview. Download preview PDF.
- Echalier, G. and Ohanessian, A. (1969) Isolement, en cultures in vitro, de lignèes cellulaires deploïdes de Drosophila melanogaster. C.R. Acad. Sci., D-268, 1771–1773.Google Scholar
- Elgin, S.C.R.E. and Miller, D.W (1978) Mass rearing of flies and mass production and harvesting of embryos. In: Ashburner, M. and Wright, T.R.F (eds) The Genetics and Biology of Drosophila, Vol. 2a. Academic Press, New York, pp 112–121.Google Scholar
- Gateff, E., Gissmann, L., Shrestha, R., Plus, N., Pfister, H., Schröder, J. and Zur Hausen, H. (1980) Characterization of two tumorous blood cell lines of Drosophila melanogaster and the viruses they contain. In: Kurstak, E., Maramorosch, K. and Dübendorfer, A. (eds) Invertebrate Systems In Vitro. Elsevier/North-Holland Biomedical, Amsterdam, pp 517–533.Google Scholar
- Kitamura, S. (1965) The in vitro cultivation of tissues from the mosquito, Culex pipiens var. molestus. II. An improved culture medium useful for ovarian tissue culture. Kobe J. Med. Sci. 11, 23–30.Google Scholar
- Kuno, G. (1981) A method for isolating continuous cell lines from Toxorhynchites amhoinensis (Diptera: Culicidae). J. Med. Entomol. 18, 140–144.Google Scholar
- Mitsuhashi, J. (1981) A new continuous cell line from larvae of mosquito Aedes albopictus (Diptera, Culicidae). Biomed. Res. 2, 63–72.Google Scholar
- Singh, K.R.P. (1967) Cell culture derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36, 506–508.Google Scholar