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Large-Scale Cell Culture

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)

Abstract

The large-scale culture of cells requires the optimization of various factors, such as cell density, cell viability, growth stage, cell nutrition, composition of media, dissolved oxygen concentration (DO), temperature, pH, and osmotic pressure of the media. In addition, the cost of the media is important for industrialization, and in this context, whether the media can be sterilized by heating is important.

Keywords

Insect Cell Bioreactor Culture Perfusion Culture Spinner Flask Insect Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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