Large-Scale Cell Culture

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


The large-scale culture of cells requires the optimization of various factors, such as cell density, cell viability, growth stage, cell nutrition, composition of media, dissolved oxygen concentration (DO), temperature, pH, and osmotic pressure of the media. In addition, the cost of the media is important for industrialization, and in this context, whether the media can be sterilized by heating is important.


Insect Cell Bioreactor Culture Perfusion Culture Spinner Flask Insect Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ackermann, M., Hellenbroich, D.H.J., and Jäger, V. (1994) Improvement of the performance of commercially available insect cell culture media for the baculovirus-directed production of recombinant proteins in bioreactors. Cytotechnology 14 (Suppl. 1), 2–8.Google Scholar
  2. Agathos, S.N., Jeong, Y.-H. and Venkat, K. (1989) Growth kinetics of free and immobilized insect cell cultures. Ann. N.Y. Acad. Sci. 97, 372–398.Google Scholar
  3. Bavarian, R, Fan, L.S. and Chalmers, J.J (1991) Microscopic visualization of insect cell-bubble interactions. I. Rising bubbles, air-medium interface, and the foam layer. Biotechnol. Prog. 7, 140–150.PubMedCrossRefGoogle Scholar
  4. Bédard, C, Kamen, A., Tom, R. and Massie, B. (1994) Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures. Cytotechnology 15, 129–138.PubMedCrossRefGoogle Scholar
  5. Chattopadhyaya, D., Rathman, J.F and Chalmers, J.J. (1995) The protective effects of specific medium additives with respect to bubble rupture. Biotechnol. Bioeng. 45, 473–480.CrossRefGoogle Scholar
  6. Hensler, W., Singh, V. and Agathos, S.N (1994) Sf 9 insect cell growth and galactosidase production in serum and serum-free media. Ann. N.Y. Acad. Sci. 745, 149–166.PubMedCrossRefGoogle Scholar
  7. Jäger, V. (1996) Perfusion bioreactors for the production of recombinant proteins in insect cells. Cytotechnology 20, 191–198.PubMedCrossRefGoogle Scholar
  8. Jem, K.J., Gong, T., Mullen, J. and Georgis, R. (1997) Development of an industrial insect cell culture process for large scale production of baculovirus biopesticides. In: Maramorosch, K. and Mitsuhashi, J. (eds) Invertebrate Cell Culture, Novel Direction and Biotechnology Applications. Science Publishers, Enfield, pp 173–180.Google Scholar
  9. Kamen, A.A., Tom, R.L., Caron, A.W., Chavarie, C, Massie, B. and Archambault, J. (1991) Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnol. Bioeng. 38, 619–628.CrossRefGoogle Scholar
  10. King, G.A., Daugulis, A.J., Goosen, M.F.A., Faulkner, P. and Bayly, D. (1989) Alginate concentration: a key factor in growth of temperature-sensitive baculovirus-infected insect cells in microcapsules. Biotechnol. Bioeng. 34, 1085–1091.PubMedCrossRefGoogle Scholar
  11. Koike, M. and Sato, K. (1988) Culture of insect cell lines originated from Mamestra brassicae with autoclaved serum-free medium. In: Kuroda, Y. and Kurstak, E. and Maramorosch, K. (eds) Invertebrate and Fish Tissue Culture. Japan Scientific Society, Tokyo, Springer, Berlin, pp 7–10.CrossRefGoogle Scholar
  12. Klöppinger, M., Fertig, G, Fraune, E. and Miltenburger, H.G,. (1990) Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures. Cytotechnology 4, 271–278.PubMedCrossRefGoogle Scholar
  13. Kumar, A. and Shuler, M.L (1995) Model of a split-flow airlift bioreactor for attachment-dependent, baculovirus-infected insect cells. Biotechnol. Prog. 11, 412–419.CrossRefGoogle Scholar
  14. Nguyen, B., Jarnagin, K., Williams, S. Chan, H. and Barnett, J. (1993) Fed-batch culture of insect cells: a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus system. J. Biotechnol. 31, 205–217.PubMedCrossRefGoogle Scholar
  15. Reuveny, S., Kim, Y.J., Kemp, C.W. and Shiloach, J. (1993) Effects of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures. Appl. Microbiol. Biotechnol. 38, 619–623.PubMedCrossRefGoogle Scholar
  16. Schuler, M.L., Cho, T., Wickham, T., Ogonah, O. Kool, M., Hammer, D.A. and Granados, R.R (1989) Bioreactor development for production of viral pesticides or heterologous proteins in insect cell cultures. Ann. N.Y. Acad. Sci. 97, 399–422.Google Scholar
  17. Shimoizu, H., Naka, Y. and Etou N. (1993) Factors affecting the yield of a baculovirus insect cell system by hollow-fiber culture. In: Kaminogawa, S., Ametani, A., and Hachimura, S. (eds) Animal Cell Technology: Basic and Applied Aspects, Vol. 5. Kluwer Academic Publishers, Dardrecht, 355–360.CrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Personalised recommendations