Gene Technology

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Attempts have been made to use cultured insect cells for the production of foreign proteins by stably transforming the cultured cells. For this purpose, usually the target gene and a gene that facilitates the selection of transformed cells such as antibiotics resistant gene are introduced at the same time. There are various methods to transfect cultured cells.


Insect Cell Selectable Marker Gene Calcium Chloride Solution Transient Assay Drosophila Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bourouis, M. and Jarry, B. (1983) Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance. EMBO J. 2, 1099–1104.PubMedGoogle Scholar
  2. Cherbas, L., Moss, R. and Cherbas, R. (1994) Transformation techniques for Drosophila cell lines. In: Goldstein, L. and Fyrberg, E. (eds) Methods in Cell Biology, Vol. 44. Academic, New York, pp 161–179.Google Scholar
  3. Di Nocera, P.P. and Dawid, I.B (1983) Transient expression of genes introduced into cultured cells of Drosophila. Proc. Natl Acad. Sci. USA 80, 7095–7098.PubMedCrossRefGoogle Scholar
  4. Durbin, J.E. and Fallon, A.M (1985) Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells. Gene 36, 173–178.PubMedCrossRefGoogle Scholar
  5. Echalier, G. (1997) Drosophila Cells in Culture, Academic Press, SanDiego, pp 702.Google Scholar
  6. Echalier, G. and Fourcade-Pernnet, F. (1994) Differences in transfection efficiency between Drosophila cell lines. In: Maramorosch, K. and Mcintosh, A.H. (eds) Insect Cell Biotechnology. CRC, Boca Raton, pp 141–156.Google Scholar
  7. Fallon, A.M (1986) Factors affecting polybrene-mediated transfection of cultured Aedes albopictus (Mosquito) cells. Exp. Cell Res. 166, 535–542.PubMedCrossRefGoogle Scholar
  8. Graham, F.L. and Van der Eb, A.J (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.PubMedCrossRefGoogle Scholar
  9. Jarvis, D.L., Fleming, J.G.W., Kovacs, G.R., Summers, M.D. and Guarino, L.A (1990) Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology 8, 950–955.PubMedCrossRefGoogle Scholar
  10. Kawai, S. and Nishizawa, M. (1984) New procedure for DNA transfection with polycation and dimethylsulfoxide. Mol. Cell. Biol. 4, 1172–1174.PubMedGoogle Scholar
  11. McLachlin, J.R. and Miller, L.K (1997) Stable transformation of insect cells to coexpress a rapidly selectable marker gene and an inhibitor of apoptosis. In Vitro Cell. Dev. Biol. 33A, 575–579.CrossRefGoogle Scholar
  12. Maisonhaute, C. and Echalier, G. (1986) Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene. FEBS Lett. 197, 45–49.PubMedCrossRefGoogle Scholar
  13. Malone, R.W., Feigner, P.L., and Verma, I.M. (1989) Cationic liposome-mediated RNA transfection. Proc. Natl Acad. Sci. USA 86, 6077–6081.Google Scholar
  14. Sinclair, J.H., Sang, J.H., Burke, J.F and Ish-Horowicz, D. (1983) Extra-chromosomal replication of copia-based vectors in cultured Drosophila cells. Nature 306, 198–200.CrossRefGoogle Scholar
  15. Van der Straten, A., Johansen, H., Sweet, R. and Rosenberg, M. (1988) Efficient expression of foreign genes in cultured Drosophila melanogaster cells using hygromycin B selection. In: Kuroda, Y., Kurstak, E. and Maramorosch, K. (eds) Invertebrate and Fish Tissue Culture. Springer, New York, 131–134.CrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Personalised recommendations