In early cell fusion studies, viruses were mostly used as fusogens. There are many viruses that produce polynucleated cells. Of the insect viruses, iridoviruses and densonucleosis virus are known to induce cell fusion. Hemagglutinating virus of Japan (HVJ) was first used as a fusogen in mammalian cell culture. This virus can also be used as a fusogen for cultured insect cells. The following procedure is based on the report of Zepp et al. (1971) on interphylum hybrid formation.
KeywordsCell Fusion Hybrid Cell Parent Cell Line Mosquito Cell Chamber Medium
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