General Cell Culture Methods

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Trypsin is the most widely used enzyme in mammalian cell culture. However, it cannot be used for the culture of some insect cells because of their sensitivity to it. The action of trypsin is inhibited by the presence of Mg2+ and Ca2+. Therefore, trypsin should be dissolved in a physiological solution free of Mg2+ and Ca2+. Rinaldini’s solution (Rinaldini, 1959; see Appendix 1) is often used as one such solution. Balanced salt solution (BSS) or phosphate-buffered saline (PBS) from which Mg2+ and Ca2+ have been omitted can also be used. The optimum pH for trypsin is 8–9. Trypsin is often used with ethylenediamine tetraacetic acid (EDTA).


Stainless Steel Mesh Continuous Cell Line Cytoplasmic Projection Programmable Freezer Liquid Nitrogen Freezer 
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© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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