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Karyotype Analysis

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)

Abstract

The karyotype is one of the characteristics of a cell line. However, it is not necessarily the same as that of cells in vivo. During culture, the karyotype of cells often changes. Consequently, many continuous cell lines are polyploid or heteroploid. Usually, the chromosome number is distributed over a wide range. This pattern of distribution may change with passages. Therefore, the karyotype is one characteristic of a continuous cell line, but discrimination of cell lines according to karyotype is not possible, especially among cell lines derived from taxonomically close species. Nevertheless, the determination of karyotype is a basic requirement for describing continuous cell lines.

Keywords

Cover Glass Pasteur Pipette Karyotype Analysis Giemsa Stain Close Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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