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Cell Cloning

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)

Abstract

Most continuous insect cell lines consist of a heterogeneous cell population, probably because the cell lines originate from explants that themselves consist of various tissues or cell types. In addition, it is true that a cell line becomes heterogeneous with repeated passaging. However, it is desirable to have a more homogeneous cell population for use as experimental material. To this end, cell cloning is useful. However, cloned insect cell lines often become heterogeneous again within a short period of time. In this case, recloning or repeated cloning may help to unify the cell population. Cloning is essentially a method of isolating single cells and proliferating the isolated cells. There are several methods by which clones can be obtained.

Keywords

Cell Cloning Feeder Layer Pasteur Pipette Nurse Cell Bottom Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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