Most continuous insect cell lines consist of a heterogeneous cell population, probably because the cell lines originate from explants that themselves consist of various tissues or cell types. In addition, it is true that a cell line becomes heterogeneous with repeated passaging. However, it is desirable to have a more homogeneous cell population for use as experimental material. To this end, cell cloning is useful. However, cloned insect cell lines often become heterogeneous again within a short period of time. In this case, recloning or repeated cloning may help to unify the cell population. Cloning is essentially a method of isolating single cells and proliferating the isolated cells. There are several methods by which clones can be obtained.
KeywordsCell Cloning Feeder Layer Pasteur Pipette Nurse Cell Bottom Area
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- Itoh, T. and Mitsuhashi, J. (1995) Effects of various conditioned media on proliferation of an isolated single cell from insect cell lines. In Vitro Cell. Dev. Biol. 32A, 814–816. McIntosh, A.H. and Rectoris, C. (1974) Insect cells: colony formation and cloning in agar medium. In Vitro 10, 1–5.CrossRefGoogle Scholar
- Mitsuhashi, J. (1975) Isolation of a clone from an insect cell line by application of a fibrin clot method. Appl. Entomol. Zool. 10, 309–310.Google Scholar