Preparation of Media

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Media consisting of a few chemicals maybe prepared by dissolving these chemicals in solution one after the other. Following is the preparation of MM medium (Mitsuhashi and Maramorosch, 1964) as an example of such media. (For the composition of the medium, see Appendix 1.)


Cellulose Nitrate Insect Cell Culture Filter Holder Laminar Flow Cabinet Medium Bottle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature 195, 788–789.PubMedCrossRefGoogle Scholar
  2. Maiorella, B., Inlow, D., Shauger, A. and Harano, D. (1988) Large-scale insect cell culture for recombinant protein production. Bio / Technology 6, 1406–1410.Google Scholar
  3. Mitsuhashi, J. (1989) Nutritional requirements of insect cells in vitro. In: Mitsuhashi, J. (ed) Invertebrate Cell System Applications, Vol. I. CRC, Boca Raton, pp 3–20.Google Scholar
  4. Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22, 435–460.Google Scholar
  5. Weiss, S.A., Smith, G.C., Kalter, S.S. and Vaughn, J.L. (1981) Improved method for the production of insect cell cultures in large volume. In Vitro 17, 495–502.CrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Personalised recommendations