• Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Oocytes cultures have been made using the medium of Heacox et al., modified Cecil’s medium, or modified TC-199 medium (see Appendix 1). The following is the method of Heacox et al. (1983) to cultivate oocytes of the polychaete Nereis virens.


Cell Sheet Coelomic Fluid Sterile Seawater Subesophageal Ganglion Antibiotic Mixture 
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© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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