Advertisement

Annelida

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)

Abstract

Oocytes cultures have been made using the medium of Heacox et al., modified Cecil’s medium, or modified TC-199 medium (see Appendix 1). The following is the method of Heacox et al. (1983) to cultivate oocytes of the polychaete Nereis virens.

Keywords

Cell Sheet Coelomic Fluid Sterile Seawater Subesophageal Ganglion Antibiotic Mixture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Battaglia, M. and Davoli, C. (1997) Long-term tissue culture of the earthworm Eisenia foetida (Annelida, Oligochaeta). In: Maramorosch, K. and Mitsuhashi, J. (eds) Invertebrate Cell Culture: Novel Directions and Biotechnology Applications. Science Publishers, Enfield, pp 261–268.Google Scholar
  2. Dietzel, I.D., Drapeau, P. and Nicholls J.G (1986) Voltage dependence of 5-hydroxytryptamine release at a synapse between identified leech neurons in culture. J. Physiol. 372, 191–205.PubMedGoogle Scholar
  3. Fretter, V. and Graham, A. (1976) A Functional Anatomy of Invertebrates. Academic Press, London, pp.589.Google Scholar
  4. Heacox, A.E., Fischer, A. and Frangenberg, H.R (1983) Development of a medium for in vitro culture of oocyte from the polychaete, Nereis virens. In Vitro 19, 825–832.CrossRefGoogle Scholar
  5. Krontowsky, A. and Rumianzew, A. (1922) Zur Technik der Gewebs-kulturen von Regenwürmern in vitro. Pflügers Arch. Gesamte Physiol. Menschen Tiere 195, 291–299.CrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Personalised recommendations