Advertisement

Prochordata

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)

Abstract

Circulating hemocytes of the solitary protochordates do not survive for prolonged periods in vitro. Subsequently, pharyngeal tissue, which is considered a hemocytopoietic tissue, has been cultured by Raftos et al. (1990). The following procedure is based on their method.

Keywords

Streptomycin Sulfate Epithelial Cell Culture Pharyngeal Tissue Copyright Owner Pyloric Cecum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Raftos, D.A., Stillman, D.L. and Cooper, E.L (1990) In vitro culture of tissue from the tunicate Styela clava. In Vitro Cell. Dev. Biol. 26, 962–970.PubMedCrossRefGoogle Scholar
  2. Rinkevich, B. and Rabinowitz, C. (1993) In vitro culture of blood cells from the colonial protochordate Botryllus schlosseri. In Vitro Cell. Dev. Biol. 29A, 79–85.CrossRefGoogle Scholar
  3. Rinkevich, B. and Rabinowitz, C. (1994) Acquiring embryo-derived cell cultures and aseptic metamorphosis of larvae from the colonial protochordate Botryllus schlosseri. Invert. Reproduct. Dev. 25, 59–72.CrossRefGoogle Scholar
  4. Rinkevich, B. and Rabinowitz, C. (1997) Initiation of epithelial cell cultures from palleal buds of Botryllus schlosseri, a colonial tunicate. In Vitro Cell. Dev. Biol. 33A, 422–424.CrossRefGoogle Scholar
  5. Stachowitsch, M. (1992) The Invertebrates. An Illustrated Glossary. Wiley-Liss, New York, pp.676.Google Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Personalised recommendations