Abstract
There have not been many attempts made to culture orthopteran cells. However, long-term primary cultures of orthopteran cells are possible, and several cell lines have been established from grasshopper cell cultures (Tsang et al., 1981). Usually, cockroach media, or a modification of these media, are used, and it has been said that a high Na+/K+ ratio in the medium is important to grow orthopteran cells (Quiot, 1971).
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References
Quiot, J.M (1971) Cell culture of Coleoptera, Orthoptera and Dictioptera. In: Vago, C. (ed) Invertebrate Tissue Culture, Vol. 1. Academic, New York, pp 267–294.
Tsang, K.R., Freeman, F.A., Kurtti, T.J. and Brooks, M.A (1981) New cell lines from embryos of Melanoplus sanguinipes (F.) (Orthoptera: Acrididae). Acrida 10, 105–111.
Vago, C, Quiot, J.M. and Luciani, J. (1968) Cultures de cellules cardiaques d’arthropodes. In: Barigozzi, C. (ed) Proceedings of the Second International Colloquium on Invertebrate Tissue Culture. Istituto Lombardo, di Scienze e Lettere Milano, pp 110–118.
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© 2002 Springer Japan
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Mitsuhashi, J. (2002). Insecta (Orthoptera). In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_11
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DOI: https://doi.org/10.1007/978-4-431-67875-5_11
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