Insecta (Orthoptera)

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


There have not been many attempts made to culture orthopteran cells. However, long-term primary cultures of orthopteran cells are possible, and several cell lines have been established from grasshopper cell cultures (Tsang et al., 1981). Usually, cockroach media, or a modification of these media, are used, and it has been said that a high Na+/K+ ratio in the medium is important to grow orthopteran cells (Quiot, 1971).


Sodium Hypochlorite Ethyl Ether Culture Vessel Embryonic Tissue Tissue Fragment 
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  1. Quiot, J.M (1971) Cell culture of Coleoptera, Orthoptera and Dictioptera. In: Vago, C. (ed) Invertebrate Tissue Culture, Vol. 1. Academic, New York, pp 267–294.CrossRefGoogle Scholar
  2. Tsang, K.R., Freeman, F.A., Kurtti, T.J. and Brooks, M.A (1981) New cell lines from embryos of Melanoplus sanguinipes (F.) (Orthoptera: Acrididae). Acrida 10, 105–111.Google Scholar
  3. Vago, C, Quiot, J.M. and Luciani, J. (1968) Cultures de cellules cardiaques d’arthropodes. In: Barigozzi, C. (ed) Proceedings of the Second International Colloquium on Invertebrate Tissue Culture. Istituto Lombardo, di Scienze e Lettere Milano, pp 110–118.Google Scholar

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© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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