Photon Emission by Natural Radical Scavengers in the Presence of Active Oxygen Species
The occurrence and intensity of photon emission were observed to be closely related to the partial structures of flavonoids. Photon emission P is suggested to be given by [P] = k [X] [Y] [Z]. The photon constants k of catechins and gallic acid in the presence of hydrogen peroxide as X and acetalde-hyde as Z were calculated from k 1 k 2, and k 3 of [P] = k 1 [X] [Y] f (z), [P] = k 2 [Y] [Z] f (x), and [P] = k 3 [X] [Z] f (y). k 1 showed greater differences depending on the structure of catechins than k 2 and k 3. The XYZ photon emission system is thought to consist of the transformation of energy. From the results of analysis by high-pressure liquid chromatography (HPLC) or antioxidative effects against lipid autoxidation, the XYZ system is suggested to be a scavenging mechanism of active oxygen species. DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponin from soybean showed as strong an effect as Z in the presence of hydrogen peroxide and phenolic compounds. The XYZ system suggests that natural radical scavengers have various combinations for synergistic inhibition of the radical reactions.
KeywordsElectron Spin Resonance Gallic Acid Photon Emission Active Oxygen Species Hydroxyl Radical Scavenge Activity
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- 1.Kelvin JAD (1987) Protein damage and degradation by oxygen radicals. J Biol Chem 262:9895–9901Google Scholar
- 19.Yoshiki Y, Kahara T, Igarashi K, Yotsuhashi K, Okubo K (1995) Chemiluminescence of oxygen radical scavengers such as DDMP saponins in the presence of radicals and aldehyde, 210th ACS National Meeting Aug. 20–24, abstract AGFD 183Google Scholar
- 22.Yoshiki Y, Kahara T, Igarashi K, Yotsuhashi K, Okubo K (1997) Chemiluminescence of catechins and gallic acid in the presence of acetaldehyde and active oxygen species, (in press)Google Scholar
- 23.Amarowicz R, Shimoyamada M, Okubo K (1994) Hypocho-lesterolemic effects of saponins. Roczn Pzh 45:125–131Google Scholar
- 25.Okubo K, Kudou S, Uchida T, Yoshiki Y, Yoshikoshi M, Tonomura M (1994) Soybean saponin and isoflavonoids: structure and antiviral activity for cancer prevention against human immunodeficiency virus in vitro. Food phytochemicals for cancer prevention I. ACS Symposium 546:330–339CrossRefGoogle Scholar
- 27.Yoshiki Y, Kudo K, Yoshikoshi M, Okubo K (1995) Chemiluminescence of soybean saponins in the presence of active oxygen species and scavenger in soybean fermented foods, International Chemical Congress of Pacific Basin Societies, Honolulu, Dec. 17–22, abstract, AGRO 1607Google Scholar
- 30.Niki E, Saito T, Kawakami A, Kamiya Y (1983) Inhibition of oxidation of methyl linoleate in solution by vitamin E and vitamin C. J Biol Chem 259:4177–4182Google Scholar