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Using BIACORE as Pots and Pans

  • Tohru Natsume

Abstract

One day a graduate student came to me with a despairing look and told me a GST fusion protein expressed in E. coli was not soluble after all his efforts. Checking the SDS-PAGE gave evidence of GST fusion protein produced in large amount, but its band was not detected in the sample of supernatant after centrifugation. It was apparent that the recombinant had formed inclusion bodies. I am sure many of you often have the same experiences.

Keywords

Recombinant Protein Graduate Student Inclusion Body Protein Sample Soluble Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Tohru Natsume

There are no affiliations available

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