Using BIACORE as Pots and Pans
One day a graduate student came to me with a despairing look and told me a GST fusion protein expressed in E. coli was not soluble after all his efforts. Checking the SDS-PAGE gave evidence of GST fusion protein produced in large amount, but its band was not detected in the sample of supernatant after centrifugation. It was apparent that the recombinant had formed inclusion bodies. I am sure many of you often have the same experiences.