Principles of BIACORE
BIACORE systems exploit surface plasmon resonance (SPR) as the detection principle to monitor the interaction between biomolecules in real time without labeling. One of the interacting partners is immobilized on the surface of a sensor chip, while the sample containing the other partner(s) is injected over the surface at a constant flow rate through a microfluidic channel system. The minute changes in mass concentrations at the surface of the sensor chip as a consequence of association and dissociation between the molecules is measured as an SPR response, and is displayed as a function of time on a graph known as sensorgram. This approach differs from many conventional methods in that it can measure interaction in a short time with a small amount of sample. For the interaction between biomolecules, the technique can measure not only the affinity at equilibrium (dissociation constant, K D, or affinity constant, K A) but also the parameters that characterize the reaction rate, that is, the association rate constant k a and dissociation rate constant k d.
KeywordsSurface Plasmon Resonance Sensor Surface Sensor Chip Resonance Unit Resonance Angle
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