Advertisement

Principles of Surface Plasmon Resonance

  • Francis Markey

Abstract

BIACORE systems monitor surface-bound molecular interactions using surface plasmon esonance (SPR) to measure refractive index changes in solution close to the surface of a sensor (up to a istance of about 500 nm from the surface). Binding of molecules to the surface as a result of biospecific interaction changes the solute concentration in the surface volume, which influences the refractive index. The resulting change in the SPR signal is followed continuously in real time, resulting in direct monitoring of the molecular interaction process. Since the detection principle relies on changes in refractive index, no radioactive, optical, or enzymatic labels are required. The technique can be used to study all kinds of molecules including proteins and protein conjugates, carbohydrates, lipids, nucleic acids, small molecules such as pharmaceuticals, metabolites and effectors, and even larger particles such as viruses and whole cells.

Keywords

Surface Plasmon Resonance Metal Layer Critical Angle Total Internal Reflection Evanescent Wave 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Thompson N L, Lagerholm B C (1997) Total internal reflection fluorescence: applications in cellular biophysics. Curr. Opin. Biotechnol. February 1997:58–64CrossRefGoogle Scholar
  2. 2.
    Otto A (1968) Excitation of nonradiative surface plasma waves in silver by the method of frustrated total reflection. Z Phys 216:398–410CrossRefGoogle Scholar
  3. 3.
    Otto A (1968) A new method for exciting non-radiative surface plasma oscillations. Phys. Stat. Sol. 26:K99–K101CrossRefGoogle Scholar
  4. 4.
    Kretschmann E, Raether H (1968) Radiative decay of nonradiative surface plasmon excited by light. Z. Naturf. 23A:2135–2136Google Scholar
  5. 5.
    Kretschmann E (1971) Die Bestimmung optischer Konstanten von metallen durch Anregung von Oberflächenplasmaschwingungen. Z. Phys. B241:313–324Google Scholar
  6. 6.
    Kovacs G (1982) Optical Excitation of Surface Plasmon-polaritons in Layered Media. In: Boardman AD (ed) Electromagnetic Surface Modes. John Wiley & Sons, New York, ppl43–200Google Scholar
  7. 7.
    Swalen J D (1986) Optical properties of Langmuir-Blodgett films. J. Mol. Electron. 2:155–181Google Scholar
  8. 8.
    Raether H (1988) Surface plasmons. Springer, Berlin/Heidelberg/New YorkGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Francis Markey
    • 1
  1. 1.Biacore ABUppsalaSweden

Personalised recommendations