The importance of carbohydrate-lectin interactions in typical multicellular organism events is becoming widely recognized. To clarify the functional roles of these interactions, it is important to evaluate not only sugar-binding specificity but also the mechanism involved. The SPR biosensor is an entirely new assay system for affinity measurements because the interaction can be monitored ki-netically on the solid phase surface. Considering that oligosaccharides usually exist as glycoconjugates and most of the interactions with sugar-recognizing molecules occur on a solid phase surface, this method seems to be quite feasible for the analyses of those interactions.
KeywordsSurface Plasmon Resonance Sensor Chip Dissociation Rate Constant Unstirred Layer Surface Plasmon Resonance Biosensor
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