DNA-DNA Interactions

  • Masanori Gotoh
  • Mariko Tosu


Recently, the requirement for gene diagnosis has increased as the number of causal genes that have been found has increased. There are ethical problems, yet gene therapy has been popular. Medical treatments have been intimately connected with genetics. Here BIACORE applications in the medical field are also described beside mismatch detection.


Sensor Chip Affinity Constant Complementary Region Gene Diagnosis Single Mismatch 


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  1. 1.
    Gotoh M, Hasegawa Y, Shinohara Y, Shimizu M, Tosu M (1995) A new approach to determine the effect of mismatches on kinetic parameters in DNA hybridization using an optical biosensor. DNA Res. 2:285–293PubMedCrossRefGoogle Scholar
  2. 2.
    Gotoh M, Hasebe M, Ohira T, Tosu M (1997) Gene diagnosis with an affinity sensor. BIACORE: principles and applications(in Japanese). Rinsho-Byori 4:224–228Google Scholar
  3. 3.
    Gotoh M (1997) Affinity sensor (BIACORE) based on surface plasmon resonance (in Japanese). Kensa-To-Gijutsu 25:785–787Google Scholar
  4. 4.
    Gotoh M, Hasebe M, Ohira T, Hasegawa Y, Shinohara Y, Sota H, Nakao J, Tosu M (1997) Rapid method for detection of point mutations using mismatch binding protein (MutS) and an optical biosensor. Genet. Anal. 14:47–50PubMedCrossRefGoogle Scholar
  5. 5.
    Gotoh M (1997) DNA analysis with a biosensor based on surface plasmon resonance (in Japanese). Igaku-No-Ayumi 183:429–432Google Scholar
  6. 6.
    Lee J S, Woodsworth M L, Latimer L J, Morgan A R (1984) Poly (pyrimidine). Poly (Purine) synthetic DNAs containing 5-methylcytosine from stable triplex at neutral pH. Nucleic Acids Res. 12:6603–6614PubMedCrossRefGoogle Scholar
  7. 7.
    Mouscadet J F, Carteau S, Goulaouic H, Subra F, Auclair C (1994) Triplex-mediated inhibition of HIV DNA integration in vitro. J. Biol. Chem. 269:21635–21638PubMedGoogle Scholar
  8. 8.
    Chan P P, Glazer P M (1997) Triplex DNA: fundamentals, advances and potential applications for gene therapy. J. Mol. Med. 75:267–282PubMedCrossRefGoogle Scholar
  9. 9.
    Bates P J, Dosanjh H S, Kumar S, Jenkins T C, Laughton C A, Neidle S (1995) Detection and kinetic studies of triplex formation by oligonucleotides using real-time biomolecular interaction analysis (BIA). Nucleic Acids Res. 23:3627–3632PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Masanori Gotoh
    • 1
  • Mariko Tosu
    • 1
  1. 1.Amersham Pharmacia Biotech K. K.Shijuku-ku, TokyoJapan

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