Abstract
Recent studies have revealed that various protein factors have important roles in gene expression. These factors are involved in transcriptional regulation not only by binding to the promoter region of DNA with sequence specificity but also by interacting with one another on DNA. It has also been revealed that some factors bind to DNA indirectly to regulate transcription. These factors, which are called cofactors, are recruited on promoter regions by protein-protein interactions. The mechanism of transcriptional regulation includes many of interactions among biomolecules.
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References
Watanabe H, Wada T, Handa H (1990) Transcription factor E4TF1 contains two subunits with different function. EMBO J. 9: 841–847
Watanabe H, Sawada J-I, Yano K, Yamaguchi K, Goto M, Handa H (1993) cDNA Cloning of Transcription Factor E4TF1 Subunits with Ets and Notch Motifs. Mol. Cell. Biol. 13: 1385–1391
Suzuki F, Goto M, Sawa C, Ito S, Watanabe H, Sawada J-I, Handa H (1998) Functional interactions of transcription factor human GA-binding protein subunits. J. Biol. Chem. 273:29302–29308
Inomata Y, Kawaguchi H, Hiramoto M, Wada T, Handa H (1992) Direct purification of multiple ATF/E4TF3 polypeptides from HeLa cell crude nuclear extracts using DNA affinity latex particles. Anal Biochem. 206: 109–114
Mori T, Nakane M, Hattori T, Matsunaga T, Ihara M, Nikaido O(1991) Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6–4)photoproduct from the same mouse immunized with ultraviolet-irradiated DNA. Photochem. Photobiol. 54: 225–232
Komatsu Y, Tsujino T, Suzuki T, Nikaido O, Ohtsuka E(1997) Antigen structural requirements for recognition by a cyclobutane thymine dimer-specific monoclonal antibody. Nucleic Acid Res. 25: 3889–3894
Kobayashi H, Morioka H, Torizawa T, Kato K, Shimada I, Nikaido O, Ohtsuka E(1998) Specificities and binding rates of anti-(6–4) photoproduct antibody fragments to synthetic thymine photoproducts. J. Biochem. 123: 182–188
Morioka H, Miura H, Kobayashi H, Koizumi T, Fujii K, Asano K, Matsunaga T, Nikaido O, Ohtsuka E (1998) Antibodies specific for (6–4) DNA photoproducts: Cloning, antibody modeling and construction of a single-chain Fv derivative. Biochim. Biophys. Acta 1385: 17–32
Kobayashi H, Morioka H, Nikaido O, Stewart JD, Ohtsuka E (1998) The role of surface lysines in pyrimidine (6–4) pyrimidone photoproduct binding by a high-affinity antibody. Protein Eng. 11:1089–1092
Torizawa T, Kato K, Kimura Y, Asada T, Kobayashi H, Komatsu Y, Morioka H, Nikaido O, Ohtsuka E, Shimada I (1998) 31P NMR study of the interactions between oligo-deoxynucleotides containing (6–4) photoproduct and Fab fragments of monoclonal antibodies specific for (6–4) photoproduct. FEBS Lett. 429:157–161
Kobayashi H, Morioka H, Tobisawa K, Torizawa T, Kato K, Shimada I, Nikaido O, Stewart JD, Ohtsuka E (1999) Probing the Interaction between a high-affinity single-chain Fv and pyrimidine (6–4) pyrimidone photodimer by site-directed mutagenesis. Biochemistry 38:532–539
Torizawa T, Kato K, Kato J, Kobayashi H, Komatsu Y, Morioka H, Nikaido O, Ohtsuka E, Shimada I (1999) Conformational multiplicity of the antibody combining site of a monoclonal antibody specific for a (6–4) photoproduct. J. Mol. Biol. 290:731–740
Torizawa T, Yamamoto N, Suzuki T, Nobuoka K, Komatsu Y, Morioka H, Nikaido O, Ohtuka E, Kato K, Shimada I (2000) DNA binding mode of the Fab fragment of a monoclonal antibody specific for cyclobutane pyrimidine dimer. Nucleic Acids Res. 28:944–951
Borrebaeck C A(eds)(1995)Antibody engineering(second edition). Oxford University Press New York
McCafferty J, Hoogenboom H R, Chiswell D J(eds)(1996)Antibody engineering. IRL Press New York
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Sawada, Ji., Suzuki, F., Morioka, H., Kobayashi, H., Ohtsuka, E. (2000). DNA-Protein Interactions. In: Nagata, K., Handa, H. (eds) Real-Time Analysis of Biomolecular Interactions. Springer, Tokyo. https://doi.org/10.1007/978-4-431-66970-8_13
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DOI: https://doi.org/10.1007/978-4-431-66970-8_13
Publisher Name: Springer, Tokyo
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