Scleroplasty Using the Explant with Trypsin Inhibitor
A new synthetic explant with trypsin inhibitor was elaborated for enhCancement of the sclera. Experimental studies were conducted in 10 rabbits aged 2 months. Enhancement of the sclera was performed in the right eye using the explant with trypsin inhibitor and in the left eye without the inhibitor. After the operation there was no toxic, cancerogenic action of the explant with trypsin inhibitor in the surrounding tissues. The inflammatory mixed conjunctival reaction lasted 3–5 days. Electron microscopy was conducted after 2–3 weeks and at 1, 2, and 6 months following the operation. Two months after the action of the trypsin inhibitor disappeared, histologic specimens of the animal sclera showed that the graft’s threads had been invaded by thin collagenous structures of fibrins and fibroblasts with epithelioid cells; and 1 year later the sclera was regularly thickened, replacing the resolved transplant material. For the same scleral enhancement without trypsin inhibitor, there were separate connective tissue bundles with graft remnants and chaotically situated fibroblasts. Thus trypsin inhibitor leads to uniform spreading of scleral elements with simultaneous resolution of the transplant material.