Localization of Heparin-Like Compounds in Cultured Aortic Endothelial Cells
The non-thrombogenic properties of blood vessels are due, in part, to anticoagulant heparin-like compounds on the luminal surface of vascular endothelial cells. A certain class of heparan sulfate proteoglycans (HSPGs) associated with the endothelial surface can bind antithrombin III (AT III) in plasma and can accelerate the thrombin inactivation by the protease inhibitor. HSPGs are also known to be located along the abluminal side of the endothelium, namely basement membrane or extracellular matrix (ECM) produced by endothelial cells1. Thus, questions exist regarding the localization of anticoagulant HSPG in endothelial cells. Does ECM contain HSPGs which interact with antithrombin III? If they exist, how abundant are they, as compared to those in the luminal surface?
KeywordsHeparan Sulfate Chondroitin Sulfate Anti Thrombin Luminal Surface Aortic Endothelial Cell
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