Conversion of Fibrinogen to Fibrin by Thrombin and Adsorption of Thrombin by Fibrin Clots — the Observations and a Hypothesis
Thrombin (EC 22.214.171.124), a serine protease, generated by proteolytic cleavage of prothrombin (Factor II) through the concerted action of several enzymes and cofactors (1) has many effects on the hemostatic system including conversion of fibrinogen to fibrin (Fig. 1), activa-tion of Factors V, VIII, XIII, and protein C, and stimulation of platelet aggregation and release. Early studies on thrombin reaction with fibrinogen showed decreased thrombin concentration in the clot supernatant (2). The amount of thrombin lost was later found to be directly proportional to the concentration of fibrinogen (3), and also depended upon the initial concentration of thrombin (4). Kinetic studies (5) indicated that fibrin formation followed pseudo-first order kinetics with respect to fibrinogen.
KeywordsFibrin Clot Clotting Activity Scatchard Plot Hemostatic System Fibrin Monomer
Unable to display preview. Download preview PDF.
- 7.A National Cooperative Study (1974) J.A.M.A. 229, 1006–1613.Google Scholar