Natriuretic Peptide mRNA in SHR and WKY
Atrial natriuretic peptide (ANP) is a recently discovered hormone from the cardiac atria . The potent diuretic, natriuretic and vasorelaxant activities of ANP suggested the involvement of this cardiac hormone in the regulation of blood pressure, body fluid and electrolytes. Originally, studies using radioimmunoassay (RIA) and blot hybridization technique revealed the presence of large quantities of immunoreactive ANP (IR-ANP) and ANP messenger RNA (ANP mRNA) in the atrium but not in the ventricle [2,3]. However, following studies indicated the synthesis of ANP in various extraatrial tissues including the ventricle . Its biological actions directed much attention to the implication of ANP in hypertension. Changes of IR-ANP concentrations in plasma and tissue of hypertensive rats were investigated [5,6]. Elevated ANP mRNA levels in atria and ventricles of spontaneously hypertensive rats (SHR) were also reported . Recently, Sudoh et al. isolated and determined brain natriuretic peptide (BNP) from porcine brain, which derived from a distinct gene from ANP and possesses diuretic, natriuretic and vasorelaxant activities . This natriuretic peptide family, ANP and BNP, seems to construct a complex regulatory system in mammalian circulation. In this study, to elucidate the pathophysiological role of natriuretic peptide family in hypertension, we tried to determine IR-ANP, IR-BNP and ANP mRNA levels in SHR and Wistar Kyoto rats (WKY).
KeywordsNatriuretic Peptide Brain Natriuretie Peptide Atrial Natriuretie Peptide Natriuretic Peptide Receptor Atrial Natriuretie Peptide Level
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