Oxygen Free Radicals Inhibit Ca2+-pump Activity by Oxidizing Sulfhydryl Groups in Rat Heart Sarcolemmal Membrane

  • Masanori Kaneko
  • Akira Kobayashi
  • Noboru Yamazaki
Conference paper


Although oxygen free radicals have been implicated as mediators of cellular injury in some pathophysiological conditions like myocardial ischemia-reperfusion [1], the mechanisms of myocardial cell damage caused by oxygen free radicals are still unclear. In this regard, it should be pointed out that oxygen free radicals are known to attack molecules of major biological significance and these include phospholipids, protein, and nucleic acids [2]. Since sulfhydryl groups (SH groups) are known to regulate the membrane-bound enzyme activities in the cell [3, 4, 5, 6, 7], it is possible that the oxidation of SH groups in the membrane may lead to the depression of enzyme activities because of oxygen free radicals [8].


ATPase Activity Xanthine Oxidase Oxygen Free Radical Sulfhydryl Group Pump Activity 
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  1. 1.
    Ambrosio G, Becker LC,.Hutchins GM, Weisman HF, Weisfeldt ML (1986) Circulation 74:1424–1433PubMedCrossRefGoogle Scholar
  2. 2.
    Hammond B, Hess ML (1985) J Am Coll Cardiol 6:215–220PubMedCrossRefGoogle Scholar
  3. 3.
    Scherer NM, Deamer DW (1986) Arch Biochem Biophys 246:589–610PubMedCrossRefGoogle Scholar
  4. 4.
    Schoot BM, Schoots AFM, Depont JJHHM, Schuurmansstekhoven FMAH, Bonting SL (1977) Biochem Biophys Acta 483:181–192PubMedCrossRefGoogle Scholar
  5. 5.
    Schuurman-stekhoven FMAH, Bonting SL (1981) Physiol Rev 61:1–76Google Scholar
  6. 6.
    Yamada S, Ikemoto N (1978) J Biol Chem 253:6801–6807PubMedGoogle Scholar
  7. 7.
    Yoshida H, Tonomura Y (1976) J Biochem 79:649–654PubMedGoogle Scholar
  8. 8.
    Freeman BA, Crapo JD (1982) Lab Invest 47:412–426PubMedGoogle Scholar
  9. 9.
    Halliwell B, Gutteridge JM (1982) Advances in studies on heart metabolism. Bologna, CLUEB, pp 403–411.Google Scholar
  10. 10.
    Pitts BJR (1979) J Biol Chem 254:6232–6235PubMedGoogle Scholar
  11. 11.
    Kaneko M, Elimban V, Dhalla NS (1989) Am J Physiol 257 (Heart Circ Physiol 26):H804–H811Google Scholar
  12. 12.
    Belomo GF, Mirabelli P, Rickelmi P, Orrenius S (1983) FEBS Lett 163:136–139CrossRefGoogle Scholar
  13. 13.
    Kramer JH, Mak IT, Weglicki WB (1984) Circ Res 55:120–124PubMedCrossRefGoogle Scholar
  14. 14.
    Reeves JP, Bailey CA, Hale CC (1986) J Biol Chem 261: 4948–4955PubMedGoogle Scholar

Copyright information

© Springer Japan 1992

Authors and Affiliations

  • Masanori Kaneko
    • 1
  • Akira Kobayashi
    • 1
  • Noboru Yamazaki
    • 1
  1. 1.Third Department of Internal MedicineHamamatsu University School of MedicineHamamatsuJapan

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