Abstract
Tumor cell invasion and metastasis is a multifactorial process, which at each step may require the action, of proteolytic enzymes such as collagenase, cathepsins, plasmin, or plasminogen activators 1,2. An enzymatically inactive proenzyme form of the urokinase-type plasminogen activator (pro-uPA) is secreted by tumor cells 1,3. Cathepsin B, a cysteine-dependent protease, which is elevated in tumors, plays a regulatory role in collagen degradation, since it can convert inactive procollagenase IV to its enzymatically active form 4. We demonstrate that cathepsin B has the capacity to efficiently convert soluble or tumor cell receptor-bound pro-uPA to enzymatically active two-chain uPA. Thus, the cellular protease cathepsin B may substitute for the plasma protease plasmin in the activation of pro-uPA released by tumor cells.
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Dano K., Atrdreasen P.A., Grondahl-Hansen J., Kristensen P.I., Nielsen L.S. and Striver L. (1985) Adv. Cancer Res. 44: 139–226.
Liotta L.A., Rao C.N. and Barsky S.U. (1983) Lab. Invest. 491: 636–649.
Stoppelli M.P., Corti A., Soffientini A., Cassani G., Blasi F. and Assoian R.K. (1985) Proc. Natl. Acad. Sei. USA 82: 4939–4943.
Eeckhout Y. and Vaes G. (1977) Biochem. J. 166 21–31.
Estreicher A., Wohlwend A., Belin D., Schleuning W.D. and Vassalli J.D. (1989) J. Biol. Chem. 264: 1180–1189.
Laemmli U.K. (1970) Nature 227 680–685.
Schmitt M., Kanayama N., Henschen A., Hollrider A., Hafter R., Gulba D., Janicke F. and Graeff H. (1989) FEBS Lett. 255: 83–88.
Ichinose A., Fujikawa K. and Suyama T. (1986) J. Biol. Chem. 261: 3486–3489.
Gurewich V. and Pannell R. (1987) Blood 69: 969–972.
Krepela E., Bartek J., Skalkova D., Vicar J., Rasnick D., Taylor-Papadimit-riou J. and Hallowes R.C. (1987) J. Cell Sci. 87: 145–154.
Nishimura Y. and Kato K. (1987) Biochem. Biophys. Res. Commun. 148: 254–259.
Tandon A.T., Clark G.M., Chamness G.C., Chirgwin J.M. and McGuire W.L. (1990) New Engl. J. Med. 322: 297–302.
Markus G. (1988) Enzyme (Basel) 40: 158–172.
Janicke F., Schmitt M., Ulm K., Gossner W. and Graeff II. (1989) Lancet 11: 1149.
Duffy M., O’Grady P., Devaney D., O’Siorain L., Fennelly J.J. and Lijnen H.R. (1988) Cancer 62: 531–533.
Lijnen H.R., Van Hoef B., Nelles L. and Collen D. (1990) J. Biol. Chem. 265: 5232–5236.
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© 1992 Springer Japan
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Kobayashi, H. et al. (1992). Cathepsin B Efficiently Activets the Soluble and the Tumor Cell Receptor-Bound Form of the Proenzyme Urokinase-Type Plasminogen Activator (Pro-Upa). In: Takada, A., Budzynski, A.Z. (eds) Hemostasis and Circulation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-66925-8_20
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DOI: https://doi.org/10.1007/978-4-431-66925-8_20
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-70096-8
Online ISBN: 978-4-431-66925-8
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