Primary Polymerization Sites in the D-Domain of Human Fibrinogen
The conversion of fibrinogen into a fibrin clot is initiated by the limited thrombin proteolysis resulting in the release of fibrinopeptide A and the exposure of NHS-terminal polymerization sites “A” in the E domain of the fibrinogen molecule.’ Complementary polymerization sites “a” capable of interacting with the NHL-terminal sites have been attributed to the D domain, but their exact location in the fibrinogen molecule is uncertain.2-5 There are inconsistent preliminary data indicating that sites “a” may be present in sequences either γ96-2644 or γ266-411.5 The present studies were initiated to assess this discrepancy and identify the location of a polymerization sites “a” in the D domain.
KeywordsFibrin Clot Human Fibrinogen Polymerization Site Fibrinogen Molecule Disuccinimidyl Suberate
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