Primary Polymerization Sites in the D-Domain of Human Fibrinogen

  • C. S. Ciernievski
  • A. Z. Budzynski
Conference paper


The conversion of fibrinogen into a fibrin clot is initiated by the limited thrombin proteolysis resulting in the release of fibrinopeptide A and the exposure of NHS-terminal polymerization sites “A” in the E domain of the fibrinogen molecule.’ Complementary polymerization sites “a” capable of interacting with the NHL-terminal sites have been attributed to the D domain, but their exact location in the fibrinogen molecule is uncertain.2-5 There are inconsistent preliminary data indicating that sites “a” may be present in sequences either γ96-2644 or γ266-411.5 The present studies were initiated to assess this discrepancy and identify the location of a polymerization sites “a” in the D domain.


Fibrin Clot Human Fibrinogen Polymerization Site Fibrinogen Molecule Disuccinimidyl Suberate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Bettelheim F.R. and Bailey K. (1952) The products of the action of thrombin on fibrinogen. Biochim. Biophys. Acta 9: 578–579.PubMedCrossRefGoogle Scholar
  2. 2.
    Southan C., Thompson E., Panico M., Etienne T., Morris H.R. and Lane D.A. (1985) Characterization oi peptides cleaved by plasmin from the C-terminal polymerization domain of human fibrinogen. J. Biol. Chem. 260: 13095–13101.PubMedGoogle Scholar
  3. 3.
    Varadi A. and Scheraga H.A. (1986) Localization of segments essential for polymerization and for calcium binding in the γ-chain of human fibrinogen. Biochemistry 25: 519–528.PubMedCrossRefGoogle Scholar
  4. 4.
    Kuyas C., Sigrist H. and Straub P.W. (1987) Localization of fibrin polymerization sites by photoaffinity. Thromb. Haemost. 58: 287 (abstract).Google Scholar
  5. 5.
    Shimuzu A. and Doolittle R.F. (1989) Identification of fibrin polymerization site by photoaffinity labeling. Thromb. Haemost. 62: 70 (abstract).Google Scholar
  6. 6.
    Cierniewski C.S. , Kloczewiak M. and Budzynski A.Z. (1986) Expression of primary polymerization sites in the D domain of human fibrinogen depends on intact conformation. J. Biol. Chem. 261: 9116–9121.PubMedGoogle Scholar
  7. 7.
    Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.PubMedCrossRefGoogle Scholar
  8. 8.
    Laudano A.P., Cottrell B.A. and Doolittle R.F. (1983) Synthetic peptides modelled on fibrin polymerization sites. Ann. N.Y. Acad. Sci. 408: 315–329.PubMedCrossRefGoogle Scholar
  9. 9.
    D’Souza S.E. , Ginsberg M.H. , Burke T.A. and Plow E.F. (1990) The ligand binding site of the platelet integrin receptor GPIIb-IIIa is proximal to the second calcium binding domain of its α subunit. J. Biol. Chem. 265: 3440–3446.PubMedGoogle Scholar
  10. 10.
    Strong D.D., Moore M., Cottrell B.A., Bohonus V.I. , Evans B., Riley M. and Doolittle R.F. (1985) Lamprey fibrinogen γ chain: cloning, cDNA sequencing and general characterization. Biochemistry 24: 92–101.PubMedCrossRefGoogle Scholar
  11. 11.
    Procyk R. and Blomback B. (1987) Role of disulphide bonds near the calcium binding sites in fibrinogen. Thromb. Haemost. 58: 38 (abstract).Google Scholar
  12. 12.
    Bantia S., Mane S.M. , Bell W.R. and Dang C.V. (1990) Fibrinogen Baltimore I: Polymerization defect associated with a γ292Gly→Val (GGC→GTC) mutation. Blood 76: 2279–2283.PubMedGoogle Scholar
  13. 13.
    Bantia S., Bell W.R. and Dang D.V. (1990) Polymerization defect of fibrinogen Baltimore III due to a γAsn30 -→Ile mutation. Blood 75: 1659–1663.PubMedGoogle Scholar
  14. 14.
    Muramatsu S., Mimuro J., Mackawa H., Sakata Y., Matsuda M., Yoshitake S., Okuma M. (1991) Gene analysis of abnormal fibrinogens characterized by a mutation in the γ chain. Thromb. Haemost. 65: 821 (abstract).Google Scholar

Copyright information

© Springer Japan 1992

Authors and Affiliations

  • C. S. Ciernievski
    • 1
  • A. Z. Budzynski
    • 2
  1. 1.Department of BiophysicsMedical School in LodzPoland
  2. 2.Department of BiochemistryTemple University School of MedicinePhiladelphiaUSA

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