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Immune-related genes of the Japanese flounder, Paralichthys olivaceus

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Aquatic Genomics

Summary

Fish have many nonspecific and specific immune mechanisms, with humoral and cellular mechanisms to resist infectious diseases. However, the biodefense and immune-related systems of fish are poorly understood at the molecular level. We have isolated many putative biodefense and immuno response-related genes from the Japanese flounder, Paralichthys olivaceus; these include cytokines (interleukin-1β, tumor necrosis factor, chemokines, etc), cytokine receptors (TNFR-1 and −2, IL1R, IL6R, chemokine receptors, etc.), cell surface molecules (T-cell receptors, IgM, IgD, CD3, CD8, toll-like receptors, etc.), transcription factors (IRFs, CEBP, Stat3, etc.), antimicrobial proteins (lysozymes, transferrin, Mx), proteases, and protease inhibitors. We have determined the complete sequences of several biodefense and immuno response-related genes that were originally isolated as partial fragments from expressed sequence tag (EST) analysis. We have also found several virus-, bacterial endotoxin-, and mitogen-induced genes in Japanese flounder leukocytes. Some of these genes have no similarity to previously reported genes or peptides in DNA/protein databases. The existence of unidentified genes that are upregulated by viruses, bacterial endotoxins, or mitogens in fish suggested that fish have specific immune and defense mechanisms.

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Authors and Affiliations

  1. Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries, Konan 4-5-7, 108-8477, Minato-ku, Tokyo, Japan

    Ikuo Hirono & Takashi Aoki

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  1. Ikuo Hirono
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  2. Takashi Aoki
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Editors and Affiliations

  1. Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, Shinjuku-ku, Japan

    Nobuyoshi Shimizu Ph.D. (Professor and Head) (Professor and Head)

  2. Laboratory of Genetics and Biochemistry, Graduate School of Fisheries Science, Tokyo University of Fisheries, 4-5-7 Konan, 108-8477, Minato-ku, Tokyo, Japan

    Takashi Aoki Ph.D. (Professor) & Ikuo Hirono Ph.D. (Associate Professor) (Professor) &  (Associate Professor)

  3. Tokyo University of Fisheries, 4-5-7 Konan, 108-8477, Minato-ku, Tokyo, Japan

    Fumio Takashima Ph.D. (President) (President)

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Hirono, I., Aoki, T. (2003). Immune-related genes of the Japanese flounder, Paralichthys olivaceus . In: Shimizu, N., Aoki, T., Hirono, I., Takashima, F. (eds) Aquatic Genomics. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65938-9_26

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  • DOI: https://doi.org/10.1007/978-4-431-65938-9_26

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