Summary
To study osmotic stress response of fish cells, we screened osmotic stress-inducible mRNA in a common carp cell line (EPC) by RNA arbitrarily primed polymerase chain reaction and isolated a cDNA encoding a Na+- and Cl+- dependent highhigh affinity taurine transporter as a hyperosmotic stress-inducible gene. Northern blot analysis revealed the marked increase in taurine transporter mRNA by hyperosmotic stress. To study changes in taurine transporter gene expression in an individual fish, we measured the mRNA level in Mozambique tilapia (Oreochromis mossambicus) during high-salinity adaptation. By the transfer of tilapia cultured in freshwater to 70% artificial seawater, plasma osmolality increased by as much as 100–135 mOsm/kg H2O along with the marked increase in taurine transporter mRNA level in all the tissues examined. Since an upregulation of taurine transporter expression leads to the activation of accumulation of taurine in the cells, these results suggest the significant role of taurine as an osmolyte in fish.
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Takeuchi, K., Toyohara, H. (2003). Taurine transporter: hyperosmotic stress-responsive gene. In: Shimizu, N., Aoki, T., Hirono, I., Takashima, F. (eds) Aquatic Genomics. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65938-9_18
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DOI: https://doi.org/10.1007/978-4-431-65938-9_18
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