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Modulation of Vascular Smooth Muscle Contraction by Calponin Phosphorylation

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Regulation of the Contractile Cycle in Smooth Muscle

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Summary

Calponin is an abundant thin filament-associated smooth muscle protein that has been implicated in the modulation of smooth muscle contraction. Recently, we found that smooth muscle calponin is an excellent substrate for protein kinase C and that phosphorylation reduced the binding of calponin to F-actin and tropomyosin. We found that Thr184 is the preferred site of phosphorylation and is functionally the most important of the sites phosphorylated by protein kinase C in smooth muscle calponin. We have demonstrated that calponin can be rapidly dephosphorylated by type lδ protein phosphatase, just as the 20kDa light chain in intact myosin can be dephosphorylated in vitro. We investigated calponin phosphorylation during endothelin-1 or phorbol 12,13-dibutylate (PDBu) stimulation of intact strips of porcine coronary artery. Stimulation by endothelin-1 or PDBu resulted in a significant increase of 32P incorporation into the calponin in association with the development of force. These results suggest that calponin phosphorylation by protein kinase C plays a potential role in the modulation of smooth muscle contraction by agonists.

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Authors and Affiliations

  1. Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie, 514, Japan

    Toshio Tanaka, Michiko Naka, Terumasa Mino, Uhito Yuasa, Fumiaki Nakamura, Junichi Yamamoto & Satoshi Matsushima

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  1. Toshio Tanaka
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  2. Michiko Naka
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  3. Terumasa Mino
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  4. Uhito Yuasa
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  5. Fumiaki Nakamura
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  6. Junichi Yamamoto
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  7. Satoshi Matsushima
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Editors and Affiliations

  1. Mie University of School Medicine, 2-175 Edobashi, Tsu, Mie, 514, Japan

    Takeshi Nakano M.D., Ph.D. (Professor) (Professor)

  2. University of Arizona, 85721, Tucson, AZ, USA

    David J. Hartshorne Ph.D. (Professor) (Professor)

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© 1995 Springer-Verlag Tokyo

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Tanaka, T. et al. (1995). Modulation of Vascular Smooth Muscle Contraction by Calponin Phosphorylation. In: Nakano, T., Hartshorne, D.J. (eds) Regulation of the Contractile Cycle in Smooth Muscle. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65880-1_14

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  • DOI: https://doi.org/10.1007/978-4-431-65880-1_14

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-65882-5

  • Online ISBN: 978-4-431-65880-1

  • eBook Packages: Springer Book Archive

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