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Actin-Binding Property of Myosin Light Chain Kinase and Its Role in Regulating Actin-Myosin Interaction of Smooth Muscle

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Regulation of the Contractile Cycle in Smooth Muscle

Summary

Myosin light chain kinase (MLCK), a key regulator of smooth muscle contraction, has a property to bind to actin filaments that is obviously unrelated to the kinase activity innate in MLCK. We have found that this property exerts an inhibitory effect on the ATP-dependent interaction between actin and myosin. The N-terminal, actin-binding fragment was responsible for the inhibition. Calmodulin in the presence of Ca2+ (Ca2+/CaM) efficiently relieved the inhibition imposed by MLCK. The inhibitory effect was potent enough to explain physiological involvement in terms of the amount of MLCK that was present as a cytoskeletal protein in smooth muscle.

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Authors and Affiliations

  1. Department of Pharmacology, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371, Japan

    Li-Hong Ye, Kohichi Hayakawa, Tsuyoshi Okagaki & Kazuhiro Kohama

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  1. Li-Hong Ye
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  2. Kohichi Hayakawa
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  3. Tsuyoshi Okagaki
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  4. Kazuhiro Kohama
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Editors and Affiliations

  1. Mie University of School Medicine, 2-175 Edobashi, Tsu, Mie, 514, Japan

    Takeshi Nakano M.D., Ph.D. (Professor) (Professor)

  2. University of Arizona, 85721, Tucson, AZ, USA

    David J. Hartshorne Ph.D. (Professor) (Professor)

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© 1995 Springer-Verlag Tokyo

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Ye, LH., Hayakawa, K., Okagaki, T., Kohama, K. (1995). Actin-Binding Property of Myosin Light Chain Kinase and Its Role in Regulating Actin-Myosin Interaction of Smooth Muscle. In: Nakano, T., Hartshorne, D.J. (eds) Regulation of the Contractile Cycle in Smooth Muscle. Springer, Tokyo. https://doi.org/10.1007/978-4-431-65880-1_10

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  • DOI: https://doi.org/10.1007/978-4-431-65880-1_10

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-65882-5

  • Online ISBN: 978-4-431-65880-1

  • eBook Packages: Springer Book Archive

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