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Three-Dimensional Structural Analyses in Cryogenic X-Ray Diffraction Imaging

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X-Ray Diffraction Imaging of Biological Cells

Part of the book series: Springer Series in Optical Sciences ((SSOS,volume 210))

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Abstract

An electron density map projected along the direction of the incident X-ray is obtained from a single small-angle diffraction pattern. However, most projection maps are difficult to interpret. The final goal of a structural analysis is a visualization of the electron density distribution in three dimensions. In this chapter, the tomography X-ray diffraction imaging (XDI) method is first introduced to visualize three-dimensional (3D) electron density maps of particles in XDI using synchrotron radiation . In XDI experiments using X-ray free electron laser (XFEL) pulses , as the specimen particles are destroyed by single X-ray pulses, tomography experiments are impossible. Under the assumption that the structures of the particles are similar at a low resolution, 3D reconstruction is possible by utilizing the single particle analysis method developed in transmission electron microscopy (TEM) . A scheme for 3D reconstruction in XFEL -XDI through simulations for macromolecules and experiments on a cellular organelle is proposed.

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Authors and Affiliations

  1. Department of Physics, Faculty of Science and Technology, Keio University, Yokohama, Kanagawa, Japan

    Masayoshi Nakasako

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  1. Masayoshi Nakasako
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Correspondence to Masayoshi Nakasako .

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Nakasako, M. (2018). Three-Dimensional Structural Analyses in Cryogenic X-Ray Diffraction Imaging. In: X-Ray Diffraction Imaging of Biological Cells. Springer Series in Optical Sciences, vol 210. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56618-2_9

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