Abstract
The technique of photoaffinity labeling has become increasingly appreciated as a powerful methodology for post-genome field because it is one of the attractive methods to elucidate the interactions between bioactive ligand and biomolecule. The combinations with detection and isolation methods are essential to identify photolabeled components. There are several methods to introduce detection and isolation methods, which are so-called “tag,” for photolabeled components from the photoaffinity label mixture. High detection limits of tag enable us to identify the photolabeled components. The introduction of detection and isolation tags in the ligand skeleton is one of the ways to archive identification of photolabeled components because the labeled components have been only introduced the tag. On the other hands, the specific biological interactions for target biomolecules are also utilized to identify photolabeled components. The chapter summarized that the several combinations of photoaffinity labeling and “tag” to study labeled components effectively.
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Acknowledgments
M.H. is very grateful to Prof. Y. Hatanaka (University of Toyama), G.D. Holman (University of Bath), and Prof. Y. Kanaoka (Toyama College) for valuable advice throughout the manuscript.
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Hashimoto, M. (2017). Multifunctional Photoprobes for Identification of Ligand Sites Within Biomolecules. In: Hatanaka, Y., Hashimoto, M. (eds) Photoaffinity Labeling for Structural Probing Within Protein. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56569-7_1
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DOI: https://doi.org/10.1007/978-4-431-56569-7_1
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