Abstract
Developmental changes in the expression and localization of drebrin has been mainly analyzed in chick embryo and young rat by various anti-drebrin polyclonal and monoclonal antibodies. Immunoblot analysis demonstrated that the adult drebrin isoform (drebrin A) is restricted to neural tissues, while the embryonic drebrin isoforms (drebrin E1 and E2 in chicken and drebrin E in mammals) are found in a wide variety of tissues. In the developing brain, drebrin E (including chicken drebrin E2) is expressed in newly generated neurons. During neuronal migration, drebrin E is distributed ubiquitously within the neurons. Once drebrin A is expressed in the developing neuron, drebrin E is no longer present within the cell soma and accumulates in the growth cone of growing processes, resulting in the cessation of neuronal migration. The limited subcellular localization of drebrin A, which is possibly regulated by a drebrin A-specific mechanism, is likely to affect the localization of drebrin E. In the adult brain, drebrin is mainly localized in dendritic spines, but in some nuclei, drebrin can be detected in neuronal somata as well as dendritic spines. The fact that the developmental changes in drebrin expression highly correlate in time with the sensitive period of visual cortical plasticity in kittens suggests that synaptic plasticity depends on drebrin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Altman J (1972) Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer. J Comp Neurol 145:353–397
Aoki C, Sekino Y, Hanamura K, Fujisawa S, Mahadomrongkul V, Ren Y, Shirao T (2005) Drebrin A is a postsynaptic protein that localizes in vivo to the submembranous surface of dendritic sites forming excitatory synapses. J Comp Neurol 483:383–402
Arai H, Sato K, Uto A, Yasumoto Y (1991) Effect of transient cerebral ischemia in mongolian gerbils on synaptic vesicle protein (SVP-38) and developmentally regulated brain protein (drebrin). Neurosci Res Commun 9:143–150
Chimura T, Launey T, Yoshida N (2015) Calpain-mediated degradation of drebrin by excitotoxicity in vitro and in vivo. PLoS One 10:e0125119
Daw NW, Fox K, Sato H, Czepita D (1992) Critical period for monocular deprivation in the cat visual cortex. J Neurophysiol 67:197–202
Hanamura K, Kamata Y, Yamazaki H, Kojima N, Shirao T (2017) Isoform-dependent regulation of drebrin dynamics in dendritic spines. Neuroscience
Imamura K, Shirao T, Mori K, Obata K (1992) Changes of drebrin expression in the visual cortex of the cat during development. Neurosci Res 13:33–41
Kojima N, Shirao T, Obata K (1993) Molecular cloning of a developmentally regulated brain protein, chicken drebrin A and its expression by alternative splicing of the drebrin gene. Brain Res Mol Brain Res 19:101–114
LaVail JH, Cowan WM (1971a) The development of the chick optic tectum. I. Normal morphology and cytoarchitectonic development. Brain Res 28:391–419
LaVail JH, Cowan WM (1971b) The development of the chick optic tectum. II. Autoradiographic studies. Brain Res 28:421–441
Mizui T, Kojima N, Yamazaki H, Katayama M, Hanamura K, Shirao T (2009) Drebrin E is involved in the regulation of axonal growth through actin-myosin interactions. J Neurochem 109:611–622
Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K (1996) Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem 66:980–988
Sharma S, Grintsevich EE, Phillips ML, Reisler E, Gimzewski JK (2011) Atomic force microscopy reveals drebrin induced remodeling of f-actin with subnanometer resolution. Nano Lett 11:825–827
Sharma S, Grintsevich EE, Hsueh C, Reisler E, Gimzewski JK (2012) Molecular cooperativity of drebrin1-300 binding and structural remodeling of F-actin. Biophys J 103:275–283
Shirao T, Obata K (1985) Two acidic proteins associated with brain development in chick embryo. J Neurochem 44:1210–1216
Shirao T, Obata K (1986) Immunochemical homology of 3 developmentally regulated brain proteins and their developmental change in neuronal distribution. Brain Res 394:233–244
Shirao T, Inoue HK, Kano Y, Obata K (1987) Localization of a developmentally regulated neuron-specific protein S54 in dendrites as revealed by immunoelectron microscopy. Brain Res 413:374–378
Shirao T, Kojima N, Terada S, Obata K (1990) Expression of three drebrin isoforms in the developing nervous system. Neurosci Res Suppl 13:S106–S111
Shirao T, Kojima N, Obata K (1992) Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells. Neuroreport 3:109–112
Shirao T, Hayashi K, Ishikawa R, Isa K, Asada H, Ikeda K, Uyemura K (1994) Formation of thick, curving bundles of actin by drebrin A expressed in fibroblasts. Exp Cell Res 215:145–153
Song M, Kojima N, Hanamura K, Sekino Y, Inoue HK, Mikuni M, Shirao T (2008) Expression of drebrin E in migrating neuroblasts in adult rat brain: coincidence between drebrin E disappearance from cell body and cessation of migration. Neuroscience 152:670–682
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Japan KK
About this chapter
Cite this chapter
Shirao, T., Koganezawa, N., Yamazaki, H., Hanamura, K., Imamura, K. (2017). Localization of Drebrin: Light Microscopy Study. In: Shirao, T., Sekino, Y. (eds) Drebrin. Advances in Experimental Medicine and Biology, vol 1006. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56550-5_7
Download citation
DOI: https://doi.org/10.1007/978-4-431-56550-5_7
Published:
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-56548-2
Online ISBN: 978-4-431-56550-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)