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In natural, each ARS specifically recognizes each tRNA and amino acid, and tRNAs are specifically aminoacylated with cognate amino acids by cognate ARSs. On the other hand, flexizymes, which are in vitro selected aminoacylation ribozymes, enable to aminoacylate RNAs bearing various 3′-ends with various amino acids including both canonical and noncanonical amino acids. By utilizing these unique characteristics, novel interaction between folic acid and hsa-pre-miR-125a was discovered (Chap. 2), and an orthogonal ribosome–tRNA pair was developed by engineering of peptidyl transferase center (Chap. 3). Aminoacylation at 3′-end of RNA with various amino acids by flexizymes has a great potential to be applied for various RNA studies including analysis of translation mechanisms, engineering of translation, discoveries of novel small ncRNAs.
KeywordsRibozyme MicroRNA SELEX tRNA Ribosome Translation
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