Abstract
The last decade has witnessed the rapid growth of techniques that can be used to examine a variety of questions in the cricket Gryllus bimaculatus. In this chapter, we provide detailed and well-established protocols for injections (21.1), RNAi approaches (21.2), genome editing (21.3), and gene expression analysis (21.4). These protocols can be used not only in other Orthopteran species, but also as a starting point for functional analyses in other hemimetabolous insects from Odonatans to Hemipterans.
These protocols were adapted from those developed in several labs predominantly the labs of Sumihare Noji, Aleksandar Popadić, and Cassandra Extavour.
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Horch, H., Liu, J., Mito, T., Popadić, A., Watanabe, T. (2017). Protocols in the Cricket. In: Horch, H., Mito, T., Popadić, A., Ohuchi, H., Noji, S. (eds) The Cricket as a Model Organism. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56478-2_21
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