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77Se NMR Spectroscopy for Speciation Analysis of Selenium Compounds

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Abstract

Nuclear magnetic resonance (NMR) spectroscopy is widely used for the determination of the chemical structure of an organic compound. NMR spectroscopy is theoretically applicable to all metallic elements except cerium. In this chapter, we demonstrate selenium (Se) speciation in animal Se metabolites (selenometabolites) by NMR spectroscopy and review hitherto performed NMR spectroscopic studies of Se detection in biological samples. 77Se, an NMR-active isotope of Se, was directly observed, but its NMR receptivity was lower than its sensitivity in mass spectrometry. However, the use of enriched stable isotope improved the receptivity. Each selenometabolite had its own chemical shift, suggesting that the chemical shift of 77Se could be used as a fingerprint. Indirect measurement by heteronuclear multiple bond correlation (HMBC) spectroscopy with 1H nuclides was also effective for the Se speciation.

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Acknowledgment

This work was supported by JSPS KAKENHI Grants-in-Aid for Scientific Research (Grant Numbers 26460032 (N.S.), 26293030 (Y.O.), 15 K14991 (Y.O.), and 16H05812 (Y.O.)).

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Authors and Affiliations

  1. Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba, 260-8675, Japan

    Noriyuki Suzuki Ph.D. & Yasumitsu Ogra

Authors
  1. Noriyuki Suzuki Ph.D.
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  2. Yasumitsu Ogra
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Corresponding author

Correspondence to Noriyuki Suzuki Ph.D. .

Editor information

Editors and Affiliations

  1. Graduate School of Pharmaceutical Science, Chiba University, Chiba, Japan

    Yasumitsu Ogra

  2. Graduate School of Science, The University of Tokyo, Tokyo, Japan

    Takafumi Hirata

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Suzuki, N., Ogra, Y. (2017). 77Se NMR Spectroscopy for Speciation Analysis of Selenium Compounds. In: Ogra, Y., Hirata, T. (eds) Metallomics. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56463-8_7

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