Abstract
The regeneration of functional immune organ will be one of major focus in future immunology research. It will be an useful tool which induces efficient immune responses in the body on demand and offers effective ways to restore the immune status and treat uncontrollable obstinate diseases such as cancer, autoimmune diseases, severe infection and immuno-insufficiency/deficiency caused by tissue damages, abnormality, primary defect and aging. Artificially synthesized lymphoid organs may also provide us with a highly informative method not only for clinical aim but also basic study on the development and functions of immunological tissues and organs. We first reported successful generation of artificially-constructed lymph node-like tertiary lymphoid tissues at ectopic sites in mouse by applying certain stromal cell lines (Suematsu S, Watanabe T. Nat Biotechnol 22(12):1539–1545, 2004; Okamoto N et al. J Clin Invest 117(4):997–1007, 2007; Kobayashi Y, Watanabe T. Trends Immunol 31(11):422–428, 2010). They showed a remarkable ability to induce immune responses upon antigen stimulation, especially when transplanted into naïve or immune-compromised hosts. In this review, we discuss about the rationale and method for the synthesis of functional tertiary lymph node-like lymphoid tissues in mouse. Especially, we discuss here on the method with applying only soluble factors but without using any stromal cell, that enables proper accumulation and functional organization of immune cells in grafts.
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Abbreviations
- SLO:
-
secondary lymphoid organs
- TLO:
-
tertiary lymphoid organs
- LN:
-
lymph node
- PP:
-
Payer’s patches
- DC:
-
dendritic cells
- FDC:
-
follicular dendritic cells
- FRC:
-
fibroblastic reticular cells
- HEV:
-
high endothelial venules
- RIP- LTα:
-
rat insulin promotor expressing Lta (lymphotoxin-α) gene
- LTi:
-
lymphoid tissue inducer
- LTo:
-
lymphoid tissue organizer
- LTRβ:
-
lymphotoxin receptor-beta
- RA:
-
retinoic acid
- VEGFc:
-
vessel endothelial growth factor
- Aire:
-
autoimmune regulator
- MDSCs:
-
myeloid-derived suppressor cells
- Treg cells:
-
regulatory T cells
- HSPGs:
-
heparan sulfate proteoglycans
- HS:
-
heparan sulfate
- TES:
-
tissue-engineered spleen
- GC:
-
germinal center
- aAPC:
-
artificial antigen-presenting cells
- aLN:
-
artificial lymph nodes
- iPS:
-
induced pluripotent stem cells
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This work was supported by the Grant-in-Aid for Scientific Research on Priority Areas from MEXT, Japan (Grant No. 24111009)
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Kobayashi, Y., Kato, K., Nakamura, M., Watanabe, T. (2016). Synthesis of Functional Tertiary Lymphoid Organs. In: Watanabe, T., Takahama, Y. (eds) Synthetic Immunology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56027-2_7
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