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The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension

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Analytical Ultracentrifugation

Abstract

The multiwavelength (MWL) detector is a new type of absorption detector for AUC. The commercial absorption detector of the Beckman Coulter XL-A AUC can only handle a single wavelength per scan with the possibility to scan at maximum 3 wavelengths, whereas MWL-AUC can handle all the wavelengths in the UV/Vis region at one time. The result is impressive since now a full spectral dimension is added to each single scan. In this chapter, we are explaining development history, instrumentation, and future perspective of MWL-AUC.

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Notes

  1. 1.

    Please note that besides MWL, also the abbreviation MWA for multiwavelength absorption detector is used in the literature. Both abbreviations are equivalent.

  2. 2.

    Note that the light can only be made parallel for one wavelength due to the wavelength dependence of the refractive index (chromatic aberration).

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Acknowledgments

We thank a number of people who helped to make MWL-AUC what it is today. First of all, we acknowledge funding of the AUC 2004 project by BASF SE, which made the start of this project possible. Dr. Walter Mächtle, Dr. Lars Börger, and Dr. Wendel Wohlleben, all from BASF SE, are thanked for the fruitful cooperation over the years of development. Dipl. Ing. Johannes Walter (University of Erlangen) is thanked for the new data acquisition software. We also thank Dr. Kristian Schilling (Nanolytics) for discussions and exchange of ideas during detector development. Prof. Dr. Borries Demeler (University of Texas, Health Center, at San Antonio) is thanked for adapting the UltraScan evaluation software to MWL data, which now allows unsurpassed evaluations using MWL-AUC as well as for a long-standing cooperation in this area. We also thank all students and coworkers who worked and work in the Cölfen lab on MWL development, namely, Patricyja Maciejewska, Saroj Bhattacharyya, Akif Gülsün, Basri Cicek, Dirk Haffke, Joe Pearson, Dr. Holger Strauss, and Dr. Karel Planken.

Author information

Authors and Affiliations

  1. Chemistry Department, Izmir Institute of Technology, TR-35430, Izmir, Turkey

    Engin Karabudak

  2. Department of Chemistry, University of Konstanz, Universitätsstr. 10, D-78457, Constance, Germany

    Helmut Cölfen

Authors
  1. Engin Karabudak
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  2. Helmut Cölfen
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Corresponding author

Correspondence to Helmut Cölfen .

Editor information

Editors and Affiliations

  1. Graduate School of Engineering, Osaka University, Suita, Osaka, Japan

    Susumu Uchiyama

  2. Life Science Research Center, Nihon University, Fujisawa, Kanagawa, Japan

    Fumio Arisaka

  3. Department of Neurology, Harvard Medical School, Boston, Massachusetts, USA

    Walter F. Stafford

  4. BITC, University of New Hampshire, Durham, New Hampshire, USA

    Tom Laue

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Karabudak, E., Cölfen, H. (2016). The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension. In: Uchiyama, S., Arisaka, F., Stafford, W., Laue, T. (eds) Analytical Ultracentrifugation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55985-6_5

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