Advertisement

The Application of Mammalian Cells for Assessment of Metallic Products (In Vitro Process)

  • Akiko Ogawa
Chapter

Abstract

This chapter describes the use of mammalian cells to evaluate environmental friendliness in regards to biological responses, metallic toxicity, etc. It discusses several in vitro assays using mammalian cell cultures to measure some features of metallic products. Information is provided about both the colony formation assay and the viable cell number assay for detecting changes in surface conditions of several metallic materials. It should be noted that this type of detection is difficult using traditional analyzing techniques including conventional chemical analyses, scientific analyses of materials with various microscopes, etc. Being compared with conventional methods, the biological methods with a mammalian cell culture system (described in this chapter) could make it possible to quantify the effect of environmental factors such as the components of alloys and heavy metals and to easily realize the relationship between them and organisms.

Keywords

Colony Formation Assay Hexavalent Chromium Mammalian Cell Culture Viable Cell Number Inductively Couple Plasma Atomic Emission Spectrometer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

Some parts of these works were supported by KAKENHI (21760600: Grant-in-Aid for Young Scientists (B) of 2009–2010; 24710035: Grant-in-Aid for Young Scientists (B) of 2012–2013; 26550084: Grant-in-Aid for Exploratory Research of 2015–2016).

References

  1. 1.
    da Frausto Silva JJR, Williams RJP (2001) The biological chemistry of the elements – the inorganic chemistry of life, 2nd edn. Oxford University Press, OxfordGoogle Scholar
  2. 2.
    Sakurai H, Kinzoku ha naze jintai ni hitsuyouka (Japanese). Kodansha, 1996Google Scholar
  3. 3.
    Nema R, Khare S (2012) An animal cell culture: advance technology for modern research. Adv Biosci Biotechnol 3:219–226CrossRefGoogle Scholar
  4. 4.
    Landecker H (2007) Culturing life: how cells became technologies. Harvard University Press, Cambridge, MACrossRefGoogle Scholar
  5. 5.
    Fundamental techniques in cell culture. Laboratory handbook, 2nd ednGoogle Scholar
  6. 6.
    Haase H, Maret W (2010) Chapter 8: The regulatory and signaling functions of zinc ions in human cellular physiology. In: Zalups RK, Koropatnick J (eds) Cellular and molecular biology of metals. CRC Press, Boca RatonGoogle Scholar
  7. 7.
    Kanbe T (2011) An overview of a wide range of functions of ZnT and Zip zinc transporters in the secretory pathway. Biosci Biotechnol Biochem 75:1036–1043CrossRefGoogle Scholar
  8. 8.
    Garrick MD, Dolan KG, Horbinski G, Ghio AJ, Higgins D, Porubcin M, Moore EG, Hainsworth LN, Umbreit JN, Conrad M e, Feng L, Lis A, Roth FA, Singleton S, Garrick LM (2003) DMTI: a mammalian transporter for multiple metals. Bio Met 16:41–54Google Scholar
  9. 9.
  10. 10.
    Voltrova S, Hidasova D, Genzer J, Srogl J (2011) Metallothionein-inspired prototype of molecular pincer. Chem Cmommun 47:8067–8069CrossRefGoogle Scholar
  11. 11.
    Vasak M, Mcloni G (2011) Chemistry and biology of mammalian metallothioneins. J Biol Inorg Chem 16:1067–1078CrossRefGoogle Scholar
  12. 12.
    Cousins RJ (1994) Metal elements and gene expression. Ann Rev Nutr 14:449–469CrossRefGoogle Scholar
  13. 13.
    Coyle P, Philcox JC, Carey LC, Rofe AM (2002) Metallothionein: the multipurpose protein, CMLS. Cell Mol Life Sci 59:627–647CrossRefGoogle Scholar
  14. 14.
    Ogawa A, Okuda N, Kanematsu H (2009) Comparison of biological toxicity of several plating products by mammalian cells, 2009 Sur/Fin Technical Conference Proceeding, Louisville, Kentucky, USA, June 15–17, National Association for Surface Finishing (NASF), Surface Finishing Research Session, CD-ROMGoogle Scholar
  15. 15.
    Kanematsu H, Murata K, Ogawa A, Miyano Y (2008) Sn-Zn alloy films produced by HSSL and their environmental characteristics, 2008 Sur/Fin Technical Conference Proceeding, NASF, Indianapolis, Indiana, USA, June 16–18, Process and Environmental Session, CD-ROMGoogle Scholar
  16. 16.
    Kanematsu H, Kobayashi T, Oki T, Method for producing a tin-zinc alloy film. Japan patent JP, 3438–000030, JGoogle Scholar
  17. 17.
    Ogawa A, Okuda N, Hio K, Kanematsu H, Tamauchi H (2012) The monitoring possibility of some mammalian cells for zinc concentrations on metallic materials. Cytotechnology 64:341–347CrossRefGoogle Scholar
  18. 18.
    Ogawa A, Akatsuka R, Tamauchi H, Hio K, Kanematsu H (2011) Influence of nickel-titanium alloy components on biological functions. BMC Proc 5:79CrossRefGoogle Scholar

Copyright information

© Springer Japan 2016

Authors and Affiliations

  1. 1.National Institute of Technology, Suzuka CollegeSuzukaJapan

Personalised recommendations