The Application of Mammalian Cells for Assessment of Metallic Products (In Vitro Process)
This chapter describes the use of mammalian cells to evaluate environmental friendliness in regards to biological responses, metallic toxicity, etc. It discusses several in vitro assays using mammalian cell cultures to measure some features of metallic products. Information is provided about both the colony formation assay and the viable cell number assay for detecting changes in surface conditions of several metallic materials. It should be noted that this type of detection is difficult using traditional analyzing techniques including conventional chemical analyses, scientific analyses of materials with various microscopes, etc. Being compared with conventional methods, the biological methods with a mammalian cell culture system (described in this chapter) could make it possible to quantify the effect of environmental factors such as the components of alloys and heavy metals and to easily realize the relationship between them and organisms.
KeywordsColony Formation Assay Hexavalent Chromium Mammalian Cell Culture Viable Cell Number Inductively Couple Plasma Atomic Emission Spectrometer
Some parts of these works were supported by KAKENHI (21760600: Grant-in-Aid for Young Scientists (B) of 2009–2010; 24710035: Grant-in-Aid for Young Scientists (B) of 2012–2013; 26550084: Grant-in-Aid for Exploratory Research of 2015–2016).
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