Abstract
Lipids as well as proteins, nucleic acids, and carbohydrates are essential components of animals and plants, and their chemical structures and biological functions are highly variable. However, most lipids are insoluble in aqueous solutions, and careful manipulation using organic solvents is required for the extraction and purification of lipids. In this chapter, we describe basic techniques for the extraction and purification of lipids from animal tissues and cells using the Bligh and Dyer method, solvent fractionation, and column chromatography. General methods for the extraction and purification of lipids are mentioned here; refer to other chapters for detailed information on individual lipids.
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Okuno, T., Yokomizo, T. (2015). Basic Techniques for Lipid Extraction from Tissues and Cells. In: Yokomizo, T., Murakami, M. (eds) Bioactive Lipid Mediators. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55669-5_23
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DOI: https://doi.org/10.1007/978-4-431-55669-5_23
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-55668-8
Online ISBN: 978-4-431-55669-5
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