Abstract
Signal transduction systems are known to regulate complex biological events such as cell proliferation and differentiation via sequential phosphorylation/dephosphorylation reactions over all cellular networks. Recent technological advances regarding high-resolution mass spectrometry-based quantitative proteomics, in combination with phosphorylation-directed protein/peptide enrichment methodology, have enabled us to grasp the comprehensive status of phosphorylated cellular signaling molecules in a time-resolved manner. Phosphotyrosine-targeted sample enrichment by anti-phosphotyrosine antibodies allows us to describe key regulatory signaling dynamics triggered by tyrosine kinases, including epidermal growth factor receptor, in various contexts of cancer cell signaling. Furthermore, chemistry-based phosphopeptide enrichment technologies such as immobilized metal affinity chromatography and metal oxide chromatography lead us to obtain a serine/threonine/tyrosine-phosphorylation dependent global landscape of cellular signaling at the network level. In this chapter, we introduce recent technological advances regarding phosphoproteomics-based computational analyses of signaling regulation and discuss the future directions of cancer research toward theoretical exploration of drug targets from a system-level point of view.
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Acknowledgments
We gratefully acknowledge our colleagues at Medical Proteomics Laboratory, the Institute of Medical Science, the University of Tokyo for helpful discussions and comments. This work was supported by Grants-in-Aid for Scientific Research on Innovative Areas from Japan Society for the Promotion of Science (JSPS) and The Ministry of Education, Culture, Sports, Science and Technology (MEXT).
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Kozuka-Hata, H., Oyama, M. (2015). Phosphoproteomics-Based Network Analysis of Cancer Cell Signaling Systems. In: Inoue, Ji., Takekawa, M. (eds) Protein Modifications in Pathogenic Dysregulation of Signaling. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55561-2_1
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DOI: https://doi.org/10.1007/978-4-431-55561-2_1
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