Abstract
Pancreatic fistula (PF) remains the most serious complication of digestive surgery. The primary reason for PF not being successfully averted is that there are no techniques that identify pancreatic leak from the pancreatic stump during surgery or that rapidly evaluate protease activities in leakage fluid, which can cause severe tissue damage. To improve the safety of pancreatic surgery, the development of a novel technique that would identify pancreatic leak during surgery has been anticipated. We have designed and developed a novel fluorescence probe (glutaryl phenylalanine hydroxymethyl rhodamine green) that is activated by chymotrypsin in pancreatic juice. We evaluated the probe’s ability to identify a pancreatic leak and directly sprayed it on the pancreatic stump in an animal experiment; the real-time gross visualization of a pancreatic leak was confirmed.
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Abbreviations
- PF:
-
pancreatic fistula
- ICG:
-
indocyanine green
- gPhe-HMRG:
-
glutaryl phenylalanine hydroxymethyl rhodamine green
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Acknowledgment
This work was supported by grants from the Takeda Science Foundation; the Mochida Memorial Foundation for Medical and Pharmaceutical Research; the Ministry of Education, Culture, Sports, Science and Technology of Japan; and the Ministry of Health, Labour and Welfare of Japan.
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Yamashita, S., Ishizawa, T., Kamiya, M., Urano, Y., Kokudo, N. (2016). Fluorescence Imaging for Intraoperative Identification of Pancreatic Leak. In: Kusano, M., Kokudo, N., Toi, M., Kaibori, M. (eds) ICG Fluorescence Imaging and Navigation Surgery. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55528-5_34
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DOI: https://doi.org/10.1007/978-4-431-55528-5_34
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