Anchorage-Dependent Multicellular Aggregate Formation Induces CD44 High Cancer Stem Cell-Like Phenotypes in Adult T Cell Leukemia/Lymphoma Cells
Adult T cell leukemia/lymphoma (ATL) is a highly invasive and intractable T cell malignancy caused by human T cell leukemia virus-1 infection. Leukemia/lymphoma cells that have invaded the tissues exhibit a propensity for strong resistance to chemotherapy, presenting a major obstacle to the treatment of ATL patients. Therefore, understanding how tissue-infiltrating leukemia/lymphoma cells acquire intractable features is important for developing effective treatments for ATL patients. We have recently found that, when co-cultured with epithelial-like feeder cells, ATL cells form anchorage-dependent multicellular aggregates and that a fraction of aggregate-forming ATL cells acquire quiescent CD44 high cancer stem cell-like phenotypes. This observation suggests that the intractability of tissue-infiltrating ATL cells may be partly accounted for by the acquisition of cancer stem cell-like properties.
KeywordsAdult T-cell leukemia Cancer stem cells CD44 Coculture Multicellular aggregates
This work was supported in part by Grants-in-Aid for Scientific Research from The Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).
Competing Financial Interests
The authors declare no competing financial interests.
- Furukawa Y, Osame M, Kubota R et al (1995) Human T-cell leukemia virus type-1 (HTLV-1) Tax is expressed at the same level in infected cells of HTLV-1-associated myelopathy or tropical spastic paraparesis patients as in asymptomatic carriers but at a lower level in adult T-cell leukemia cells. Blood 85(7):1865–1870PubMedGoogle Scholar