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Watercore in Fruits

  • Akihiro ItaiEmail author
Chapter
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Abstract

Watercore is a physiological internal disorder affecting apples and pears, in which the intercellular air spaces of the flesh become filled with liquid, resulting in tissues with translucent appearance. Watercore is associated with fruit maturity as well as the presentation of varietal differences in susceptibility in apples and pears. Susceptibility is thus considered a heritable character. Watercore is promoted by low or high air temperatures during the preharvest period, large fruit, poor calcium concentration, high nitrogen and boron nutrition, a high leaf-to-fruit ratio, excessive fruit thinning, high or low light exposure, growth in volcanic ash soil, ethrel (ethephon) and gibberellin treatment, and girdling of the trunk and limbs. Mild watercore symptoms can disappear in storage, but when severe, internal browning and large cavities can develop. The fleshy tissue of apples with watercore has a higher sorbitol and sucrose concentration and lower glucose concentration than tissue without watercore. Watercore is also accompanied by changes in membrane permeability during maturation and ripening. A decrease in the expression of sorbitol transporter, leading to sorbitol accumulation in the intercellular spaces and subsequent flooding of tissues, has also been suggested.

Keywords

Apple Membrane integrity Pear Sink–source relationship Sorbitol Sugar transporter 

References

  1. Abe K, Sato Y, Saito T, Kurihara A, Kotobuki K (1995) Narrow-sense heritability of fruit characters in Japanese pear (Pyrus pyrifolia Nakai). Breed Sci 45:1–5Google Scholar
  2. Amezquita R, Dewey DH (1971) Fruit internal breakdown relative to sorbitol content in ‘Jonathan’ apples. HortScience 6:280Google Scholar
  3. Aomori Apple Experiment Station (1953) Results of the apple breeding work. Bull Apple Exp Sta 4:103Google Scholar
  4. Atkinson JD (1971) Diseases of tree fruits in New Zealand. NZ Dept Sci Ind Res Inf Ser 81:170–173Google Scholar
  5. Ballard WS, Magness JR, Hawkins LA (1922) Internal browning of the ‘Yellow Newton’ apple. US Dept Agric Bull 1104Google Scholar
  6. Bangerth F (1973) Investigations upon Ca-related physiological disorders. Phytopathol Z 77:20–37CrossRefGoogle Scholar
  7. Baranowski P, Lipecki J, Mazurek W, Walczak RT (2008) Detection of watercore in ‘Gloster’ apples using thermography. Postharvest Biol Technol 47:358–366CrossRefGoogle Scholar
  8. Bartram R (1969) Use of Alar on ‘Red Delicious’ in 1968. Goodfruit Grower 19:11–13Google Scholar
  9. Bieleski RL (1977) Accumulation of sorbitol and glucose by leaf slices of Rosaceae. Aust J Plant Physiol 4:11–24CrossRefGoogle Scholar
  10. Bir RE, Bramlage WJ (1972) Responses of ‘Richared Delicious’ apple fruit to freezing temperature. II. Whole fruit tests. HortScience 7:324Google Scholar
  11. Bowen JH, Watkins CB (1997) Fruit maturity, carbohydrate and mineral content relationships with watercore in ‘Fuji’ apples. Postharvest Biol Technol 11:31–38CrossRefGoogle Scholar
  12. Bramlage WJ, Thompson AH (1962) The effects of early-season sprays of boron on fruit set, color, finish, and storage life of apples. Proc Am Soc Hortic Sci 80:64–72Google Scholar
  13. Brown PH, Hu H (1996) Phloem mobility of boron is species dependent: evidence for phloem mobility in sorbitol-rich species. Ann Bot 77:497–505CrossRefGoogle Scholar
  14. Burg SP, Burg EA, Marks R (1964) Relationship of soluble leakage to tonicity in fruits and other plant tissues. Plant Physiol 39:185–191PubMedCentralPubMedCrossRefGoogle Scholar
  15. Carne WM, Martin D (1934) Apple investigations in Tasmania: miscellaneous notes. J Council Sci Ind Res 7:207–214Google Scholar
  16. Cavallieri R (1997) Detection of watercore in apples. Washington State Univ Tree Fruit Postharvest J 8:3–8Google Scholar
  17. Chagné D, Crowhurst RN, Pindo M, Thrimawithana A, Deng C, Ireland H, Fiers M, Dzierzon H, Cestaro A, Fontana P, Bianco L, Lu A, Storey R, Knäbel M, Saeed M, Montanari S, Kim YK, Nicolini D, Larger S, Stefani E, Allan AC, Bowen J, Harvey I, Johnston J, Malnoy M, Troggio M, Perchepied L, Sawyer G, Wiedow C, Won K, Viola R, Hellens RP, Brewer L, Bus VGM, Schaffer RJ, Gardiner SE, Velasco R (2014) The draft genome sequence of European pear (Pyrus communis L. ‘Bartlett’). PLoS One 9:e92644yGoogle Scholar
  18. Cho BK, Chayaprasert W, Stroshine RL (2008) Effects of internal browning and watercore on low field (5.4 MHz) proton magnetic resonance measurements of T2 values of whole apples. Postharvest Biol Technol 47:81–89CrossRefGoogle Scholar
  19. Chun JP, Tamura F, Tanabe K, Itai A (2003a) Physiological and chemical changes associated with watercore development induced by GA in Japanese pear ‘Akibae’ and ‘Housui’. J Jpn Soc Hortic Sci 72:378–384CrossRefGoogle Scholar
  20. Chun JP, Tamura F, Tanabe K, Itai A, Tabuchi T (2003b) Cell wall degradation and structural changes of GA-induced watercored tissues in Japanese pear ‘Akibae’ and ‘Hosui’. J Jpn Soc Hortic Sci 72:488–496CrossRefGoogle Scholar
  21. Clark CJ, MacFall JS, Bieleski RL (1998) Loss of watercore from ‘Fuji’ apple observed by magnetic resonance imaging. Sci Hortic 73:213–227CrossRefGoogle Scholar
  22. Couey HM, Wiliams MW (1973) Preharvest application of ethephon on scald and quality of stored ‘Delicious’ apples. HortScience 8:56–57Google Scholar
  23. Drazeta L, Lang A, Hall AJ, Volz RK, Jameson PE (2004) Air volume measurement of ‘Braeburn’ apple fuit. J Exp Bot 55:1061–1069PubMedCrossRefGoogle Scholar
  24. Ferguson IB, Watkins CB (1989) Bitter pit in apple fruit. Hortic Rev 11:289–355Google Scholar
  25. Filder JC, Wilkinson BG, Edney KL, Sharples RO (1973) The biology of apple and pear storage. Research review no. 3. Commonwealth Bureau of Horticulture and Plantation Crops. Commonwealth Agricultural Bureau, East Malling, EnglandGoogle Scholar
  26. Fukuda H (1977) Effect of calcium on the incidence of internal breakdown of ‘Delicious’. Bull Fruit Tree Res Sta (Morioka) Series C 4:13–23Google Scholar
  27. Gao Z, Jayanty S, Beaudry R, Loesher W (2005) Sorbitol transporter expression in apple sink tissues: implications for sugar accumulation and watercore development. J Am Soc Hortic Sci 130:261–268Google Scholar
  28. Gemma H, Oomori S, Sugaya S, Peng SA, Iwahori S (2002) Study on watercore occurrence in ‘Hosui’ Japanese pear. Acta Hortic 596:845–850Google Scholar
  29. Greene DW, Lord WJ, Bramlage WJ (1977) Mid-summer application of ethephon and daminozide on apples. II. Effect on ‘Delicious.’ J Am Soc Hortic Sci 102:494–497Google Scholar
  30. Harker FR, Watkins CB, Brookfield PL, Miller MJ, Reid S, Jackson PJ, Bieleski RL, Bartley T (1999) Maturity and regional influences on watercore development and its postharvest disappearance in Fuji apples. J Am Soc Hortic Sci 124:166–172Google Scholar
  31. Harley CP (1938) Some associated factors in the development of watercore. Proc Am Soc Hortic Sci 36:435–438Google Scholar
  32. Hayama H, Iwatani A, Nishimoto T, Oya Y, Nakamura Y (2014) Watercore disorder in Japanese pear ‘Niitaka’ is increased by high fruit temperatures during fruit maturation. Sci Hortic 175:27–32CrossRefGoogle Scholar
  33. Hu H, Brown PH (1994) Localization of boron in cell wall of squash and tobacco and its association with pectin. Plant Physiol 105:681–689PubMedCentralPubMedGoogle Scholar
  34. Iida M, Bantog NA, Yamada K, Shiratake K, Yamaki S (2004) Sorbitol- and other sugar-induced expressions of the NAD+-dependent sorbitol dehydrogenase gene in Japanese pear fruit. J Am Soc Hortic Sci 129:870–875Google Scholar
  35. Inomata Y, Murase S, Nagara M, Shinokawa T, Oikawa S, Suzuki K (1993a) Studies on factors which reduce watercore in Japanese pear (Pyrus pyrifolia Nakai cv. Hosui). J Jpn Soc Hortic Sci 62:257–266CrossRefGoogle Scholar
  36. Inomata Y, Murase S, Nagara M, Shinokawa T, Suzuki K (1993b) Relationship between watercore and membrane permeability in the Japanese pear (Pyrus pyrifolia Nakai cv. Hosui). J Jpn Soc Hortic Sci 62:267–275CrossRefGoogle Scholar
  37. Inomata Y, Oikawa S, Yaegaki H, Suzuki K (1996) Effect of gibberellins and their synthesis inhibitors on the occurrence of watercore in ‘Hosui’ Japanese pear. Bull Fruit Tree Res Sta 29:51–65Google Scholar
  38. Inomata Y, Yaegaki H, Suzuki K (1999) The effects of polyethylene bagging, calcium carbonate treatment and difference in fruit–air temperatures on the occurrence of watercore in Japanese pear ‘Housui’. J Jpn Soc Hortic Sci 68:336–342CrossRefGoogle Scholar
  39. Jackson JE (2003) Biology of apples and pears. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  40. Kajiura I (1994) Nashi (Japanese pear). In: Konishi K, Iwahori S, Kitagawa H, Yukawa T (eds) Horticulture in Japan. Asakura, Tokyo, pp 40–47Google Scholar
  41. Kajiura I, Sato Y (1990) Recent progress in Japanese pear (Pyrus pyrifolia Nakai) breeding and descriptions of cultivars based on literature review. Bull Fruit Tree Res Sta Extr 1:1–329Google Scholar
  42. Kajiura I, Yamaki S, Omura M, Shimura I (1976) Watercore in Japanese pear (Pyrus serotina Rehder var. ‘Culta’ Rehder). I. Description of the disorder and its relation to fruit maturity. Sci Hortic 4:261–270CrossRefGoogle Scholar
  43. Kanayama Y (2009) Physiological roles of polyols in horticultural crops. J Jpn Soc Hortic Sci 78:158–168CrossRefGoogle Scholar
  44. Kanayama Y, Mori H, Imaseki H, Yamaki S (1992) Nucleotide sequence of a cDNA encoding NADP-sorbitol-6-phosphate dehydrogenase from apple. Plant Physiol 100:1607–1608PubMedCentralPubMedCrossRefGoogle Scholar
  45. Kim S, Schatzki TF (2000) Apple watercore sorting system using X-ray imagery. I. Algorithm development. Trans ASAE 43:1695–1702CrossRefGoogle Scholar
  46. Kollas DA (1968) Physiology of watercore development in apple. Ph.D. Thesis, Cornell University, Ithaca, NYGoogle Scholar
  47. Lakso AN (1994) Apple. In: Schaffer BS, Andersen PC (eds) Handbook of environmental physiology of fruit crops, vol 1. CRC, Boca Raton, pp 3–42Google Scholar
  48. Loescher WH (1987) Physiology and metabolism of sugar alcohols in higher plants. Physiol Plant 70:553–557CrossRefGoogle Scholar
  49. Marlow GC, Loescher W (1984) Watercore. Hortic Rev 6:189–251Google Scholar
  50. Marlow GC, Loescher W (1985) Sorbitol metabolism, the climacteric, and watercore in apples. J Am Soc Hortic Sci 110:676–680Google Scholar
  51. Moriguchi T, Abe K, Sanada T, Yamaki S (1992) Levels and role of sucrose synthase, sucrose-phosphate synthase, and acid invertase in sucrose accumulation in fruit of Asian pear. J Am Soc Hortic Sci 117:274–278Google Scholar
  52. O’Loughlin JB, Matthews CD (1968) Maturity studies of ‘Delicious’ apples 165/1967. Tasmanian J Agric 39:109–112Google Scholar
  53. Palmer RC (1931) Recent progress in the study of Jonathan breakdown in Canada. Sci Hortic 11:243–258Google Scholar
  54. Perring MA (1968) Mineral composition of apples. VIII. Further investigations into the relationship between composition and disorders of the fruits. J Sci Food Agric 19:640–645CrossRefGoogle Scholar
  55. Perring MA (1971) Watercore in apples. Annu Rep East Malling Res Sta 1973:161–162Google Scholar
  56. Perring MA, Pearson K (1979) Mineral composition and the occurrence of disorders. Annu Rep East Malling Res Sta 1978:149Google Scholar
  57. Perring MA, Majoyeogbe RA, Pearson K (1974) Watercore in relation to fruit composition. Annu Rep East Malling Res Sta 1973:149Google Scholar
  58. Sakuma F, Umeya T, Tahira K, Katagiri S, Hiyama H (1995) Effects of high temperature and/or gibberellins treatments during early fruit development on the occurrence of watercore in Japanese pear (Pyrus pyrifolia Nakai cv. Hosui). J Jpn Soc Hortic Sci 64:243–249CrossRefGoogle Scholar
  59. Sakuma F, Katagiri S, Tahira K, Umeya T, Hiyama H (2000) Effects of high temperature and/or controlling transpiration by bagging and/or spraying an anti-transpirant on the occurrence of watercore in Japanese pear ‘Housui’ (Pyrus pyrifolia Nakai). J Jpn Soc Hortic Sci 69:283–289CrossRefGoogle Scholar
  60. Schatzki TF, Haff RP, Young R, Can I, Le LC, Toyofuku N (1997) Defect detection in apples by means of X-ray imaging. Trans ASAE 40:1407–1415CrossRefGoogle Scholar
  61. Sharples RO (1967) A note on the occurrence of watercore breakdown apples during 1966. Plant Pathol 16:11–120CrossRefGoogle Scholar
  62. Siddiqui S, Bangerth F (1993) Studies on cell wall-mediated changes during storage calcium-infiltrated apples. Acta Hortic 326:105–113Google Scholar
  63. Smith AJM (1937) Anatomy of the apple-fruit. Rep Food Invest Board Dept Sci Indus Res Great Britain 127–133Google Scholar
  64. Tamura F, Chun JP, Tanabe K, Morimoto M, Itai A (2003) Effect of summer-pruning and gibberellin on the watercore development in Japanese pear ‘Akibae’ fruit. J Jpn Soc Hortic Sci 75:372–377CrossRefGoogle Scholar
  65. Tanaka K, Inomata Y, Kawase S, Sekimoto Y, Nagamura K, Kawakami C (1992) The physiological mechanism of watercore in Japanese pear (Pyrus pyrifolia Nakai var. culta Nakai) and its prevention by calcium EDTA. J Jpn Soc Hortic Sci 61:183–190Google Scholar
  66. Tanase K, Yamaki S (2000) Sucrose synthase isozymes related to sucrose accumulation during fruit development of Japanese pear (Pyrus pyrifolia Nakai). J Jpn Soc Hortic Sci 69:671–676CrossRefGoogle Scholar
  67. Terakami S, Kimura T, Nishitani C, Sawamura Y, Saito T, Hirabayashi T, Yamamoto T (2009) Genetic linkage map of the Japanese pear ‘Housui’ identifying three homozygous genomic regions. J Jpn Soc Hortic Sci 78:417–424CrossRefGoogle Scholar
  68. Throop JA, Aneshansley DJ, Upchurch BL (1994) Camera system effects on detecting watercore in ‘Red Delicious’ apples. Trans ASAE 37:873–877CrossRefGoogle Scholar
  69. Tomana T, Yamada H (1988) Relationship between temperature and fruit quality of apple cultivars grown at different locations. J Jpn Soc Hortic Sci 56:391–397CrossRefGoogle Scholar
  70. Tong YA, Zhou HJ, Yang RL, Zhang GF (1980) Inorganic constituents and nitrogen content of Delicious apples in relation to the occurrence of water core on calcareous soils. Sci Agric Sin 2:67–71Google Scholar
  71. Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A, Fontana P, Bhatnagar SK, Troggio M, Pruss D, Salvi S, Pindo M, Baldi P, Castelletti S, Cavaiuolo M, Coppola G, Costa F, Cova V, Dal Ri A, Goremykin V, Komjanc M, Longhi S, Magnago P, Malacarne G, Malnoy M, Micheletti D, Moretto M, Perazzolli M, Si-Ammour A, Vezzulli S, Zini E, Eldredge G, Fitzgerald LM, Gutin N, Lanchbury J, Macalma T, Mitchell JT, Reid J, Wardell B, Kodira C, Chen Z, Desany B, Niazi F, Palmer M, Koepke T, Jiwan D, Schaeffer S, Krishnan V, Wu C, Chu VT, King ST, Vick J, Tao Q, Mraz A, Stormo A, Stormo K, Bogden R, Ederle D, Stella A, Vecchietti A, Kater MM, Masiero S, Lasserre P, Lespinasse Y, Allan AC, Bus V, Chagné D, Crowhurst RN, Gleave AP, Lavezzo E, Fawcett JA, Proost S, Rouzé P, Sterck L, Toppo S, Lazzari B, Hellens RP, Durel CE, Gutin A, Bumgarner RE, Gardiner SE, Skolnick M, Egholm M, Van de Peer Y, Salamini F, Viola R (2010) The genome of the domesticated apple (Malus × domestica Borkh.). Nat Genet 42:833–839PubMedCrossRefGoogle Scholar
  72. Wade NL, Campbell LC, Bishop DG (1980) Tissue permeability and membrane composition of ripening banana fruits. J Exp Bot 31:975–982CrossRefGoogle Scholar
  73. Wang SY, Faust M (1992a) Ethylene biosynthesis and polyamine accumulation in apples with watercore. J Am Soc Hortic Sci 117:133–138Google Scholar
  74. Wang SY, Faust M (1992b) Variation in lipid composition of apples in relation to watercore. J Am Soc Hortic Sci 117:829–833Google Scholar
  75. Wang SY, Wang PC, Faust M (1988) Non-destructive detection of watercore in apple with nuclear magnetic resonance imaging. HortScience 24:106–109Google Scholar
  76. Westwood MN, Batjer LP, Billingsley HD (1967) Cell size, cell number and fruit density of apples as related to fruit size, position in the cluster and thinning method. Proc Am Soc Hortic Sci 91:51–62Google Scholar
  77. Williams MW (1966) Relationship of sugars and sorbitol to watercore in apples. Proc Am Soc Hortic Sci 88:67–75Google Scholar
  78. Williams MW (1969) The use of Alar on apples. Proc Wash State Hortic Assoc 1968:21–25Google Scholar
  79. Williams MW, Billingsley HD (1973) Watercore development in apple fruits as related to sorbitol levels in the tree sap and to minimum temperatures. J Am Soc Hortic Sci 98:205–207Google Scholar
  80. Wu J, Wang Z, Shi Z, Zhang S, Ming R, Zhu S, Khan MA, Tao S, Korban SS, Wang H, Chen NJ, Nishio T, Xu X, Cong L, Qi K, Huang X, Wang Y, Zhao X, Wu J, Deng C, Gou C, Zhou W, Yin H, Qin G, Sha Y, Tao Y, Chen H, Yang Y, Song Y, Zhan D, Wang J, Li L, Dai M, Gu C, Wang Y, Shi D, Wang X, Zhang H, Zeng L, Zheng D, Wang C, Chen M, Wang G, Xie L, Sovero V, Sha S, Huang W, Zhang S, Zhang M, Sun J, Xu L, Li Y, Liu X, Li Q, Shen J, Wang J, Paull RE, Bennetzen JL, Wang J, Zhang S (2013) The genome of the pear (Pyrus bretschneideri Rehd.). Genome Res 23:396–408PubMedCentralPubMedCrossRefGoogle Scholar
  81. Wunsche JN, Ferguson IB (2005) Crop load interactions in apple. Hortic Rev 31:231–290Google Scholar
  82. Yamada H, Ohmura H, Arai C, Terui M (1994) Effect of preharvest fruit temperature on ripening, sugars, and watercore occurrence in apples. J Am Soc Hortic Sci 119:1208–1214Google Scholar
  83. Yamada H, Takeuchi K, Hoshi A, Amano S (2004) Comparison of water relations in watercored and non-watercored apples induced by fruit temperature treatment. Sci Hortic 99:309–318CrossRefGoogle Scholar
  84. Yamada H, Kamio M, Amano S (2005) Varietal differences in susceptibility to early or high temperature-induced watercore in apples. J Jpn Soc Hortic Sci 74:115–120CrossRefGoogle Scholar
  85. Yamada H, Kaga Y, Amano S (2006) Cellular compartmentation and membrane permeability to sugars in relation to early or high temperature-induced watercore in apples. Sci Hortic 108:29–34CrossRefGoogle Scholar
  86. Yamaki S, Ino M (1992) Alteration of cellular compartmentation and membrane permeability to sugars in immature and mature apple fruit. J Am Soc Hortic Sci 117:951–954Google Scholar
  87. Yamaki S, Kajiura I (1983) Changes in the polysaccharides of cell wall, their constituent monosaccharides and some cell wall-degrading enzyme activities in the watercore fruit of Japanese pear (Pyrus serotina Rehder var. culta Rehder). J Jpn Soc Hortic Sci 52:250–255CrossRefGoogle Scholar
  88. Yamaki S, Moriguchi T (1989) Seasonal fluctuation of sorbitol related enzymes and invertase activities accompanying maturation of Japanese pear (Pyrus serotina Rehder var. culta Rehder) fruit. J Jpn Soc Hortic Sci 57:602–607CrossRefGoogle Scholar
  89. Yamaki S, Kajiura I, Omura M, Matsuda K (1976) Watercore in Japanese pear (Pyrus serotina Rehder var. culta Rehder). II. Chemical changes in watercored tissue. Sci Hortic 4:271–277CrossRefGoogle Scholar
  90. Zhang LY, Peng YB, Pelleschi-Travier S, Fan Y, Lu YF, Lu YM, Gao XP, Shen YY, Delrot S, Zhang DP (2004) Evidence of apoplasmic phloem unloading in developing apple fruit. Plant Physiol 135:574–586PubMedCentralPubMedCrossRefGoogle Scholar
  91. Zhang C, Tanabe K, Lee U, Kang S, Tokunaga T (2009) Gibberellins and N-(2-chloro-4-pyridyl)-N′-phenylurea improve retention force and reduce water core in premature fruit of Japanese pear cv. Hosui. Plant Growth Regul 58:25–34CrossRefGoogle Scholar

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© Springer Japan 2015

Authors and Affiliations

  1. 1.Faculty of AgricultureTottori UniversityTottoriJapan

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