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CRISPR/Cas9: The Leading Edge of Genome Editing Technology

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Targeted Genome Editing Using Site-Specific Nucleases

Abstract

An RNA-guided endonuclease (RGEN), known as CRISPR/Cas9, has been dramatically changing the field of genome engineering. Because CRISPR/Cas9 is much easier to introduce than ZFNs or TALENs because of its simple construction of customized vectors targeting particular genomic loci, this epoch-making technology has rapidly become a standard tool for targeted gene modification within a time span of just a few years. In this chapter, we explain how the technology has arisen, how it has become established, improved, and applied, and how it will evolve in the future. CRISPR/Cas9-mediated genome editing strategies are likely to continue to accelerate studies on functional genomics for years to come. Moreover, nuclease-inactivated Cas9 (dCas9) with various functional domains will develop the technology to its fullest potential, in addition to ZF- and TALE-based platforms. CRISPR/Cas9 will change the face not only of genetic engineering, but also of a variety of research areas in life science studies.

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  1. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8526, Japan

    Tetsushi Sakuma & Takashi Yamamoto

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  1. Tetsushi Sakuma
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  2. Takashi Yamamoto
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Correspondence to Tetsushi Sakuma .

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  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

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Sakuma, T., Yamamoto, T. (2015). CRISPR/Cas9: The Leading Edge of Genome Editing Technology. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_2

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