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Targeted Genome Editing Using Site-Specific Nucleases pp 197–205Cite as

Genome Editing in Higher Plants

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Abstract

Genome editing with engineered nucleases (“GEEN”) has emerged as an effective genetic engineering method that uses ‘molecular scissors’—artificially engineered nucleases—to digest DNA at targeted locations in the genome of various organisms including plant species. The DNA binding domains of zinc finger (ZF) proteins were first used as plant genome editing tools via the use of designed ZF nucleases (ZFNs), with TAL-effectors (TALE) and the RNA-DNA recognition system CRISPR/Cas9 now being used as powerful genome editing tools to create targeted gene modifications, not only in model plants but also in crop species. The key to genome editing is the introduction of targeted gene-specific double-stranded DNA breaks (DSBs) using the designed endonucleases, then allowing site-directed mutagenesis via nonhomologous end joining (NHEJ) repair and/or gene targeting via homologous recombination (HR), to occur efficiently at specific sites in the genome. This chapter provides an overview of recent advances in genome editing technologies, giving an insight into current plant molecular biology and breeding techniques.

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Acknowledgments

The authors are grateful to Dr. S. Toki for his kind support to this work. This work was supported by the Grant-in-Aid for Scientific Research C from the Japan Society for the Promotion of Science (21580125 to Y.O. and 25450002 to K.O.).

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Authors and Affiliations

  1. RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

    Yuriko Osakabe

  2. Center for Collaboration among Agriculture, Industry and Commerce, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8503, Japan

    Keishi Osakabe

Authors
  1. Yuriko Osakabe
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  2. Keishi Osakabe
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Correspondence to Keishi Osakabe .

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Editors and Affiliations

  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

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© 2015 Springer Japan

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Osakabe, Y., Osakabe, K. (2015). Genome Editing in Higher Plants. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_13

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