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Engineered Nucleases Lead to Genome Editing Revolution in Rats

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Targeted Genome Editing Using Site-Specific Nucleases

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Abstract

Sequence-specific endonucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), allow simple generation of genetically modified animals. We utilized ZFNs and TALENs to generate several knockout rat models of human diseases. Furthermore, CRISPR/Cas9 used in conjunction with single-stranded oligodeoxyribonucleotides enabled us to generate several types of targeted knockin animal, such as single nucleotide polymorphism substitution, short DNA fragment integration, and large DNA fragment elimination. These powerful technologies have opened new doors for genome manipulation that enable the engineering of animal models of human disease and potential therapeutic applications.

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Authors and Affiliations

  1. Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    Kazuto Yoshimi, Takehito Kaneko, Birger Voigt & Tomoji Mashimo

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  1. Kazuto Yoshimi
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  2. Takehito Kaneko
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  3. Birger Voigt
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  4. Tomoji Mashimo
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Correspondence to Tomoji Mashimo .

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Editors and Affiliations

  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

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Yoshimi, K., Kaneko, T., Voigt, B., Mashimo, T. (2015). Engineered Nucleases Lead to Genome Editing Revolution in Rats. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_12

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