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Genome Editing in Mice Using TALENs

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Targeted Genome Editing Using Site-Specific Nucleases

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Abstract

Genetically modified animals such as knockout mice are essential for elucidating in vivo gene functions and identifying genetic contributions to the molecular pathophysiology of human diseases. For the past two decades, knockout mice have been created via embryonic stem (ES) cell-based gene targeting, a time-consuming, laborious, inefficient, and expensive process. The rapid emergence of targeted genome editing technologies is drastically revolutionizing this situation. Genome editing mediated by transcription activator-like effector (TALE) nucleases (TALENs), one of the popular genome editing tools, is a simple and powerful gene-targeting technology. With its extremely high efficiency, the mouse genome can be manipulated directly in fertilized eggs without any targeting vector or selection steps by a process called in vivo genome editing. TALEN-mediated in vivo genome editing provides an exciting opportunity for simple, convenient, and ultra-rapid production of precisely targeted knockout and knockin mice. Using this technology, researchers can freely and routinely manipulate mouse genomes and accelerate in vivo functional genomic research.

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Acknowledgements

This work was supported in part by Strategic Research Program for Brain Sciences and Grant-in-Aid for Science Research from Ministry of Education, Culture, Sports, Science and Technology of Japan, and a grant from Medical Research Institute, Tokyo Medical and Dental University. I thank K Tanaka, R Imahashi, H Ishikubo, and T Usami (Tokyo Medical and Dental University), and T Yamamoto, and T Sakuma (Hiroshima University) for technical support and useful discussions.

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  1. Laboratory of Molecular Neuroscience, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    Tomomi Aida

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  1. Tomomi Aida
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Correspondence to Tomomi Aida .

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  1. Department of Mathematical and Life Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan

    Takashi Yamamoto

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Aida, T. (2015). Genome Editing in Mice Using TALENs. In: Yamamoto, T. (eds) Targeted Genome Editing Using Site-Specific Nucleases. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55227-7_11

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