Amebiasis pp 393-408 | Cite as

Archetypical and Specialized DNA Replication Proteins in Entamoeba histolytica

  • Guillermo Pastor-Palacios
  • Varinia López-Ramírez
  • Cesar S. Cardona-Félix
  • Elisa Azuara Liceaga
  • Samuel Lara-Gonzalez
  • Luis G. Brieba


Accurate DNA replication and repair are essential tasks for survival. In eukaryotes, DNA polymerases replicate genomes that can be composed of billions of base pairs. These genomes can be chemically damaged or modified, jeopardizing its integrity, and cells have evolved mechanisms to ameliorate the mutagenic effect of DNA damage. DNA replication and DNA lesion bypass in bacteria, yeast, and humans have been widely studied; however, little is known about these processes in other organisms. Entamoeba histolytica is a parasitic protozoan responsible for amebic dysentery and hepatic abscess. Herein, we define the DNA replication apparatus of Entamoeba histolytica and review the biochemical peculiarities of family A and family B2 DNA polymerases involved in DNA lesion bypass. Our data indicate that E. histolytica is a mosaic of archetypical family B DNA polymerases (α, ε, and δ) present at the replication fork and specialized DNA polymerases with novel lesion bypass properties.


Proliferate Cellular Nuclear Antigen Entamoeba Histolytica Abasic Site TPR2 Motif Proliferate Cellular Nuclear Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Research in the LGB laboratory is supported by CONACYT-grant 128647. We thank the Howard Hughes Medical Institute for support.


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Copyright information

© Springer Japan 2015

Authors and Affiliations

  • Guillermo Pastor-Palacios
    • 1
  • Varinia López-Ramírez
    • 1
  • Cesar S. Cardona-Félix
    • 1
  • Elisa Azuara Liceaga
    • 2
  • Samuel Lara-Gonzalez
    • 3
  • Luis G. Brieba
    • 1
  1. 1.Laboratorio Nacional de Genómica para la BiodiversidadCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalIrapuatoMexico
  2. 2.Universidad Autónoma de la Ciudad de México, Posgrado en Ciencias GenómicasMéxico DFMexico
  3. 3.División de Biología MolecularIPICYT, Camino a la PresaSan Luis PotosíMexico

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